2ard
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2ard |SIZE=350|CAPTION= <scene name='initialview01'>2ard</scene>, resolution 2.600Å | |PDB= 2ard |SIZE=350|CAPTION= <scene name='initialview01'>2ard</scene>, resolution 2.600Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE DINUCLEOTIDE'>FDA</scene> | + | |LIGAND= <scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= prnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens]) | |GENE= prnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2apg|2APG]], [[2aqj|2AQJ]], [[2ar8|2AR8]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ard FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ard OCA], [http://www.ebi.ac.uk/pdbsum/2ard PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ard RCSB]</span> | ||
}} | }} | ||
| Line 29: | Line 32: | ||
[[Category: SSPF, Scottish Structural Proteomics Facility.]] | [[Category: SSPF, Scottish Structural Proteomics Facility.]] | ||
[[Category: Unversucht, S.]] | [[Category: Unversucht, S.]] | ||
| - | [[Category: FDA]] | ||
| - | [[Category: flavin-dependent halogenase]] | ||
[[Category: helical bundle]] | [[Category: helical bundle]] | ||
[[Category: sandwiched sheet]] | [[Category: sandwiched sheet]] | ||
| Line 36: | Line 37: | ||
[[Category: sspf]] | [[Category: sspf]] | ||
[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | [[Category: tryptophan 7-halogenase]] | + | [[Category: tryptophan 7-halogenase,flavin-dependent halogenase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:56:15 2008'' |
Revision as of 22:56, 30 March 2008
| |||||||
| , resolution 2.600Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | prnA (Pseudomonas fluorescens) | ||||||
| Related: | 2APG, 2AQJ, 2AR8
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination
Overview
Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.
About this Structure
2ARD is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.
Reference
Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462
Page seeded by OCA on Mon Mar 31 01:56:15 2008
Categories: Pseudomonas fluorescens | Single protein | Dong, C. | Flecks, S. | Haupt, C. | Naismith, J H. | Pee, K H.Van. | SSPF, Scottish Structural Proteomics Facility. | Unversucht, S. | Helical bundle | Sandwiched sheet | Scottish structural proteomics facility | Sspf | Structural genomic | Tryptophan 7-halogenase,flavin-dependent halogenase
