2au3
From Proteopedia
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|PDB= 2au3 |SIZE=350|CAPTION= <scene name='initialview01'>2au3</scene>, resolution 2.00Å | |PDB= 2au3 |SIZE=350|CAPTION= <scene name='initialview01'>2au3</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= dnaG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |GENE= dnaG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2au3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2au3 OCA], [http://www.ebi.ac.uk/pdbsum/2au3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2au3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Hura, G L.]] | [[Category: Hura, G L.]] | ||
[[Category: Pease, P J.]] | [[Category: Pease, P J.]] | ||
| - | [[Category: ZN]] | ||
[[Category: dna replication]] | [[Category: dna replication]] | ||
[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
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[[Category: zinc ribbon]] | [[Category: zinc ribbon]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:57:20 2008'' |
Revision as of 22:57, 30 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | dnaG (Aquifex aeolicus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Aquifex aeolicus primase (Zinc Binding and RNA Polymerase Domains)
Overview
The coordination of primase function within the replisome is an essential but poorly understood feature of lagging strand synthesis. By using crystallography and small-angle X-ray scattering (SAXS), we show that functional elements of bacterial primase transition between two dominant conformations: an extended form that uncouples a regulatory domain from its associated RNA polymerase core and a compact state that sequesters the regulatory region from the site of primer synthesis. FRET studies and priming assays reveal that the regulatory domain of one primase subunit productively associates with nucleic acid that is bound to the polymerase domain of a second protomer in trans. This intersubunit interaction allows primase to select initiation sites on template DNA and implicates the regulatory domain as a "molecular brake" that restricts primer length. Our data suggest that the replisome may cooperatively use multiple primases and this conformational switch to control initiation frequency, processivity, and ultimately, Okazaki fragment synthesis.
About this Structure
2AU3 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Crosstalk between primase subunits can act to regulate primer synthesis in trans., Corn JE, Pease PJ, Hura GL, Berger JM, Mol Cell. 2005 Nov 11;20(3):391-401. PMID:16285921
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