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3d3c

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Revision as of 21:46, 15 November 2017

Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.

Structural highlights

3d3c is a 6 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3d3b
Gene:	nusB, ssyB, b0416, JW0406 (ECOLI), rpsJ, nusE, b3321, JW3283 (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NUSB_ECOLI] One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the BoxA RNA motif. [RS10_ECOLI] Involved in the binding of tRNA to the ribosomes.[HAMAP-Rule:MF_00508]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Protein S10 is a component of the 30S ribosomal subunit and participates together with NusB protein in processive transcription antitermination. The molecular mechanisms by which S10 can act as a translation or a transcription factor are not understood. We used complementation assays and recombineering to delineate regions of S10 dispensable for antitermination, and determined the crystal structure of a transcriptionally active NusB-S10 complex. In this complex, S10 adopts the same fold as in the 30S subunit and is blocked from simultaneous association with the ribosome. Mass spectrometric mapping of UV-induced crosslinks revealed that the NusB-S10 complex presents an intermolecular, composite, and contiguous binding surface for RNAs containing BoxA antitermination signals. Furthermore, S10 overproduction complemented a nusB null phenotype. These data demonstrate that S10 and NusB together form a BoxA-binding module, that NusB facilitates entry of S10 into the transcription machinery, and that S10 represents a central hub in processive antitermination.

Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.,Luo X, Hsiao HH, Bubunenko M, Weber G, Court DL, Gottesman ME, Urlaub H, Wahl MC Mol Cell. 2008 Dec 26;32(6):791-802. PMID:19111659^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Luo X, Hsiao HH, Bubunenko M, Weber G, Court DL, Gottesman ME, Urlaub H, Wahl MC. Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex. Mol Cell. 2008 Dec 26;32(6):791-802. PMID:19111659 doi:10.1016/j.molcel.2008.10.028

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3d3c"

@@ Line 1: / Line 1: @@
 ==Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.==
 <StructureSection load='3d3c' size='340' side='right' caption='[[3d3c]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3d3b|3d3b]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nusB, ssyB, b0416, JW0406 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), rpsJ, nusE, b3321, JW3283 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d3c OCA], [http://pdbe.org/3d3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d3c RCSB], [http://www.ebi.ac.uk/pdbsum/3d3c PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d3c OCA], [http://pdbe.org/3d3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d3c RCSB], [http://www.ebi.ac.uk/pdbsum/3d3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d3c ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 3d3c" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Ribosomal protein S10|Ribosomal protein S10]]
 == References ==
 <references/>

3d3c

From Proteopedia

Revision as of 21:46, 15 November 2017

Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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