5vym

Structural highlights

Function

[BGAL_BIFAA] Involved in the hydrolysis of transgalactooligosaccharides (TOS). Highly active towards Gal(beta1-4)Gal and Gal(beta1-4)-Gal-containing oligosaccharides. Low activity towards Gal(beta1-3)Gal, lactose and Gal(beta1-3)GalOMe. No activity towards Gal(beta1-6)Gal, Gal(beta1-4)Man, Gal(alpha1-4)Gal, Gal(alpha1-3)Gal(beta1-4)Gal, lactulose, 3'fucosyllactose, lacto-N-fucopentaose I, lacto-N-fucopentaose II, cellobiose, maltose or sucrose. No transglycosylation activity is found at high substrate concentrations (100 mg/ml) and only low transglycosylation activity at lower substrate concentrations (10 mg/ml).^{[1] [2]}

References

1. ↑ Van Laere KM, Abee T, Schols HA, Beldman G, Voragen AG. Characterization of a novel beta-galactosidase from Bifidobacterium adolescentis DSM 20083 active towards transgalactooligosaccharides. Appl Environ Microbiol. 2000 Apr;66(4):1379-84. PMID:10742215
2. ↑ Hinz SW, van den Brock LA, Beldman G, Vincken JP, Voragen AG. beta-galactosidase from Bifidobacterium adolescentis DSM20083 prefers beta(1,4)-galactosides over lactose. Appl Microbiol Biotechnol. 2004 Dec;66(3):276-84. PMID:15480628 doi:http://dx.doi.org/10.1007/s00253-004-1745-9