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3l2j

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Revision as of 02:24, 16 November 2017

Dimeric structure of the ligand-free extracellular domain of the human parathyroid hormone receptor (PTH1R)

Structural highlights

3l2j is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3c4m, 3h3g
Gene:	b4034, JW3994, malE, PTHR1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The parathyroid hormone receptor (PTH1R) is a class B G protein-coupled receptor that is activated by parathyroid hormone (PTH) and PTH-related protein (PTHrP). Little is known about the oligomeric state of the receptor and its regulation by hormone. The crystal structure of the ligand-free PTH1R extracellular domain (ECD) reveals an unexpected dimer in which the C-terminal segment of both ECD protomers forms an alpha-helix that mimics PTH/PTHrP by occupying the peptide binding groove of the opposing protomer. ECD-mediated oligomerization of intact PTH1R was confirmed in living cells by bioluminescence and fluorescence resonance energy transfer experiments. As predicted by the structure, PTH binding disrupted receptor oligomerization. A receptor rendered monomeric by mutations in the ECD retained wild-type PTH binding and cAMP signaling ability. Our results are consistent with the hypothesis that PTH1R forms constitutive dimers that are dissociated by ligand binding and that monomeric PTH1R is capable of activating G protein.

Dimeric arrangement of the parathyroid hormone receptor and a structural mechanism for ligand-induced dissociation.,Pioszak AA, Harikumar KG, Parker NR, Miller LJ, Xu HE J Biol Chem. 2010 Apr 16;285(16):12435-44. Epub 2010 Feb 19. PMID:20172855^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pioszak AA, Harikumar KG, Parker NR, Miller LJ, Xu HE. Dimeric arrangement of the parathyroid hormone receptor and a structural mechanism for ligand-induced dissociation. J Biol Chem. 2010 Apr 16;285(16):12435-44. Epub 2010 Feb 19. PMID:20172855 doi:10.1074/jbc.M109.093138

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3l2j"

@@ Line 1: / Line 1: @@
 ==Dimeric structure of the ligand-free extracellular domain of the human parathyroid hormone receptor (PTH1R)==
 <StructureSection load='3l2j' size='340' side='right' caption='[[3l2j]], [[Resolution|resolution]] 3.24&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c4m|3c4m]], [[3h3g|3h3g]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b4034, JW3994, malE, PTHR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2j OCA], [http://pdbe.org/3l2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l2j RCSB], [http://www.ebi.ac.uk/pdbsum/3l2j PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2j OCA], [http://pdbe.org/3l2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l2j RCSB], [http://www.ebi.ac.uk/pdbsum/3l2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2j ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 47: / Line 48: @@
 [[Category: Membrane]]
 [[Category: Membrane protein]]
+[[Category: Periplasm]]
 [[Category: Receptor]]
 [[Category: Sugar transport]]

3l2j

From Proteopedia

Revision as of 02:24, 16 November 2017

Dimeric structure of the ligand-free extracellular domain of the human parathyroid hormone receptor (PTH1R)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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