2az3
From Proteopedia
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|PDB= 2az3 |SIZE=350|CAPTION= <scene name='initialview01'>2az3</scene>, resolution 2.20Å | |PDB= 2az3 |SIZE=350|CAPTION= <scene name='initialview01'>2az3</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CDP:CYTIDINE-5'-DIPHOSPHATE'>CDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span> |
|GENE= ndk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2242 Halobacterium salinarum]) | |GENE= ndk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2242 Halobacterium salinarum]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1nue|1NUE]], [[2az1|2AZ1]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2az3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2az3 OCA], [http://www.ebi.ac.uk/pdbsum/2az3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2az3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Tokunaga, M.]] | [[Category: Tokunaga, M.]] | ||
[[Category: Zeth, K.]] | [[Category: Zeth, K.]] | ||
| - | [[Category: CDP]] | ||
| - | [[Category: MG]] | ||
[[Category: halophilic]] | [[Category: halophilic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:59:19 2008'' |
Revision as of 22:59, 30 March 2008
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| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | ndk (Halobacterium salinarum) | ||||||
| Activity: | Nucleoside-diphosphate kinase, with EC number 2.7.4.6 | ||||||
| Related: | 1NUE, 2AZ1
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum in complex with CDP
Overview
Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties.
About this Structure
2AZ3 is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.
Reference
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum., Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D, FEBS Lett. 2005 Dec 5;579(29):6595-600. Epub 2005 Nov 9. PMID:16293253
Page seeded by OCA on Mon Mar 31 01:59:19 2008
