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Publication Abstract from PubMed

Bacterial RNA polymerase (RNAP) requires sigma factors to recognize promoter sequences. Domain 1.1 of primary sigma factors (sigma1.1) prevents their binding to promoter DNA in the absence of RNAP, and when in complex with RNAP, it occupies the DNA-binding channel of RNAP. Currently, two 3D structures of sigma1.1 are available: from Escherichia coli in complex with RNAP and from T. maritima solved free in solution. However, these two structures significantly differ, and it is unclear whether this difference is due to an altered conformation upon RNAP binding or to differences in intrinsic properties between the proteins from these two distantly related species. Here, we report the solution structure of sigma1.1 from the Gram-positive bacterium Bacillus subtilis We found that B. subtilis sigma1.1 is highly compact because of additional stabilization not present in sigma1.1 from the other two species and that it is more similar to E. coli sigma1.1. Moreover, modeling studies suggested that B. subtilis sigma1.1 requires minimal conformational changes for accommodating RNAP in the DNA channel, whereas T. maritima sigma1.1 must be rearranged to fit therein. Thus, the mesophilic species B. subtilis and E. coli share the same sigma1.1 fold, whereas the fold of sigma1.1 from the thermophile T. maritima is distinctly different. Finally, we describe an intriguing similarity between sigma1.1 and delta, an RNAP-associated protein in B. subtilis, bearing implications for the so-far unknown binding site of delta on RNAP. In conclusion, our results shed light on the conformational changes of sigma1.1 required for its accommodation within bacterial RNAP.

Solution structure of domain 1.1 of the sigmaA factor from Bacillus subtilis is preformed for binding to the RNA polymerase core.,Zachrdla M, Padrta P, Rabatinova A, Sanderova H, Barvik I, Krasny L, Zidek L J Biol Chem. 2017 Jul 14;292(28):11610-11617. doi: 10.1074/jbc.M117.784074. Epub , 2017 May 24. PMID:28539362^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.