1oks

From Proteopedia

Revision as of 14:52, 5 November 2007

CRYSTAL STRUCTURE OF THE MEASLES VIRUS PHOSPHOPROTEIN XD DOMAIN

Overview

Measles virus is a negative-sense, single-stranded RNA virus belonging to, the Mononegavirales order which comprises several human pathogens such as, Ebola, Nipah, and Hendra viruses. The phosphoprotein of measles virus is a, modular protein consisting of an intrinsically disordered N-terminal, domain (Karlin, D., Longhi, S., Receveur, V., and Canard, B. (2002), Virology 296, 251-262) and of a C-terminal moiety (PCT) composed of, alternating disordered and globular regions. We report the crystal, structure of the extreme C-terminal domain (XD) of measles virus, phosphoprotein (aa 459-507) at 1.8 A resolution. We have previously, reported that the C-terminal domain of measles virus nucleoprotein, NTAIL, is intrinsically unstructured and undergoes induced folding in the, presence of PCT (Longhi, S., Receveur-Brechot, V., Karlin, D., Johansson, K., Darbon, H., Bhella, D., Yeo, R., Finet, S., and Canard, B. (2003) J., Biol. Chem. 278, 18638-18648). Using far-UV circular dichroism, we show, that within PCT, XD is the region responsible for the induced folding of, NTAIL. The crystal structure of XD consists of three helices, arranged in, an anti-parallel triple-helix bundle. The surface of XD formed between, helices alpha2 and alpha3 displays a long hydrophobic cleft that might, provide a complementary hydrophobic surface to embed and promote folding, of the predicted alpha-helix of NTAIL. We present a tentative model of the, interaction between XD and NTAIL. These results, beyond presenting the, first measles virus protein structure, shed light both on the function of, the phosphoprotein at the molecular level and on the process of induced, folding.

About this Structure

1OKS is a Single protein structure of sequence from Measles virus with NHE as ligand. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Structure known Active Site: NHE. Full crystallographic information is available from OCA.

Reference

Crystal structure of the measles virus phosphoprotein domain responsible for the induced folding of the C-terminal domain of the nucleoprotein., Johansson K, Bourhis JM, Campanacci V, Cambillau C, Canard B, Longhi S, J Biol Chem. 2003 Nov 7;278(45):44567-73. Epub 2003 Aug 27. PMID:12944395

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