2b4h
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2b4h |SIZE=350|CAPTION= <scene name='initialview01'>2b4h</scene>, resolution 1.6Å | |PDB= 2b4h |SIZE=350|CAPTION= <scene name='initialview01'>2b4h</scene>, resolution 1.6Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= GENOME SEGMENT 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10969 Rhesus rotavirus]) | |GENE= GENOME SEGMENT 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10969 Rhesus rotavirus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1slq|1SLQ]], [[2b4h|2B4H]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b4h OCA], [http://www.ebi.ac.uk/pdbsum/2b4h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b4h RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Dormitzer, P R.]] | [[Category: Dormitzer, P R.]] | ||
[[Category: Yoder, J D.]] | [[Category: Yoder, J D.]] | ||
| - | [[Category: | + | [[Category: beta sandwich]] |
| - | [[Category: | + | [[Category: greek key]] |
| - | [[Category: | + | [[Category: membrane penetration protein]] |
| + | [[Category: non-enveloped virus]] | ||
| + | [[Category: rearrangement]] | ||
| + | [[Category: spike protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:01:26 2008'' |
Revision as of 23:01, 30 March 2008
| |||||||
| , resolution 1.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | GENOME SEGMENT 4 (Rhesus rotavirus) | ||||||
| Related: | 1SLQ, 2B4H
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Rhesus Rotavirus VP5 Antigen Domain Dimer
Overview
The spike protein VP4 is a key component of the membrane penetration apparatus of rotavirus, a nonenveloped virus that causes childhood gastroenteritis. Trypsin cleavage of VP4 produces a fragment, VP5*, with a potential membrane interaction region, and primes rotavirus for cell entry. During entry, the part of VP5* that protrudes from the virus folds back on itself and reorganizes from a local dimer to a trimer. Here, we report that a globular domain of VP5*, the VP5* antigen domain, is an autonomously folding unit that alternatively forms well-ordered dimers and trimers. Because the domain contains heterotypic neutralizing epitopes and is soluble when expressed directly, it is a promising potential subunit vaccine component. X-ray crystal structures show that the dimer resembles the spike body on trypsin-primed virions, and the trimer resembles the folded-back form of the spike. The same structural elements pack differently to form key intermolecular contacts in both oligomers. The intrinsic molecular property of alternatively forming dimers and trimers facilitates the VP5* reorganization, which is thought to mediate membrane penetration during cell entry.
About this Structure
2B4H is a Single protein structure of sequence from Rhesus rotavirus. Full crystallographic information is available from OCA.
Reference
Alternative intermolecular contacts underlie the rotavirus VP5* two- to three-fold rearrangement., Yoder JD, Dormitzer PR, EMBO J. 2006 Apr 5;25(7):1559-68. Epub 2006 Mar 2. PMID:16511559
Page seeded by OCA on Mon Mar 31 02:01:26 2008
