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4qkc

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Revision as of 13:44, 16 November 2017

Influenza A M2 wild type TM domain at low pH in the lipidic cubic phase under cryo diffraction conditions

Structural highlights

4qkc is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
NonStd Res:	,
Related:	4qk6, 4qk7, 4qkl, 4qkm
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[W8PGZ1_9INFA] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).[SAAS:SAAS00395278]

Publication Abstract from PubMed

The matrix 2 (M2) protein from influenza A virus is a proton channel that uses His37 as a selectivity filter. Here we report high-resolution (1.10 A) cryogenic crystallographic structures of the transmembrane domain of M2 at low and high pH. These structures reveal that waters within the pore form hydrogen-bonded networks or "water wires" spanning 17 A from the channel entrance to His37. Pore-lining carbonyl groups are well situated to stabilize hydronium via second-shell interactions involving bridging water molecules. In addition, room temperature crystallographic structures indicate that water becomes increasingly fluid with increasing temperature and decreasing pH, despite the higher electrostatic field. Complementary molecular dynamics simulations reveal a collective switch of hydrogen bond orientations that can contribute to the directionality of proton flux as His37 is dynamically protonated and deprotonated in the conduction cycle.

High-resolution structures of the M2 channel from influenza A virus reveal dynamic pathways for proton stabilization and transduction.,Thomaston JL, Alfonso-Prieto M, Woldeyes RA, Fraser JS, Klein ML, Fiorin G, DeGrado WF Proc Natl Acad Sci U S A. 2015 Nov 17;112(46):14260-5. doi:, 10.1073/pnas.1518493112. Epub 2015 Nov 2. PMID:26578770^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Thomaston JL, Alfonso-Prieto M, Woldeyes RA, Fraser JS, Klein ML, Fiorin G, DeGrado WF. High-resolution structures of the M2 channel from influenza A virus reveal dynamic pathways for proton stabilization and transduction. Proc Natl Acad Sci U S A. 2015 Nov 17;112(46):14260-5. doi:, 10.1073/pnas.1518493112. Epub 2015 Nov 2. PMID:26578770 doi:http://dx.doi.org/10.1073/pnas.1518493112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4qkc"

Categories: DeGrado, W F | Thomaston, J L | Ph-activated proton channel | Transmembrane alpha helix | Viral protein

@@ Line 1: / Line 1: @@
 ==Influenza A M2 wild type TM domain at low pH in the lipidic cubic phase under cryo diffraction conditions==
 <StructureSection load='4qkc' size='340' side='right' caption='[[4qkc]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qk6|4qk6]], [[4qk7|4qk7]], [[4qkl|4qkl]], [[4qkm|4qkm]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qkc OCA], [http://pdbe.org/4qkc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qkc RCSB], [http://www.ebi.ac.uk/pdbsum/4qkc PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qkc OCA], [http://pdbe.org/4qkc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qkc RCSB], [http://www.ebi.ac.uk/pdbsum/4qkc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qkc ProSAT]</span></td></tr>
 </table>
 == Function ==

4qkc

From Proteopedia

Revision as of 13:44, 16 November 2017

Influenza A M2 wild type TM domain at low pH in the lipidic cubic phase under cryo diffraction conditions

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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