5a3y

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'''Unreleased structure'''
 
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The entry 5a3y is ON HOLD until Paper Publication
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==SAD structure of Thermolysin obtained by multi crystal data collection==
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<StructureSection load='5a3y' size='340' side='right' caption='[[5a3y]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5a3y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A3Y FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a3z|5a3z]], [[5a44|5a44]], [[5a45|5a45]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a3y OCA], [http://pdbe.org/5a3y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a3y RCSB], [http://www.ebi.ac.uk/pdbsum/5a3y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a3y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Here, an automated procedure is described to identify the positions of many cryocooled crystals mounted on the same sample holder, to rapidly predict and rank their relative diffraction strengths and to collect partial X-ray diffraction data sets from as many of the crystals as desired. Subsequent hierarchical cluster analysis then allows the best combination of partial data sets, optimizing the quality of the final data set obtained. The results of applying the method developed to various systems and scenarios including the compilation of a complete data set from tiny crystals of the membrane protein bacteriorhodopsin and the collection of data sets for successful structure determination using the single-wavelength anomalous dispersion technique are also presented.
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Authors: Zander, U., Bourenkov, G., Popov, A.N., de Sanctis, D., McCarthy, A.A., Svensson, O., Round, E.S., Gordeliy, V.I., Mueller-Dieckmann, C., Leonard, G.A.
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MeshAndCollect: an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines.,Zander U, Bourenkov G, Popov AN, de Sanctis D, Svensson O, McCarthy AA, Round E, Gordeliy V, Mueller-Dieckmann C, Leonard GA Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2328-43. doi:, 10.1107/S1399004715017927. Epub 2015 Oct 31. PMID:26527148<ref>PMID:26527148</ref>
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Description: SAD structure of Thermolysin obtained by multi crystal data collection
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5a3y" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Thermolysin|Thermolysin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacillus thermoproteolyticus]]
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[[Category: Thermolysin]]
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[[Category: Bourenkov, G]]
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[[Category: Gordeliy, V I]]
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[[Category: Leonard, G A]]
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[[Category: McCarthy, A A]]
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[[Category: Mueller-Dieckmann, C]]
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[[Category: Popov, A N]]
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[[Category: Round, E S]]
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[[Category: Sanctis, D de]]
[[Category: Svensson, O]]
[[Category: Svensson, O]]
[[Category: Zander, U]]
[[Category: Zander, U]]
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[[Category: Mueller-Dieckmann, C]]
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[[Category: Hydrolase]]
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[[Category: De Sanctis, D]]
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[[Category: Multi crystal data collection]]
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[[Category: Gordeliy, V.I]]
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[[Category: Sad]]
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[[Category: Popov, A.N]]
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[[Category: Ssx]]
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[[Category: Mccarthy, A.A]]
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[[Category: Synchrotron serial crystallography]]
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[[Category: Bourenkov, G]]
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[[Category: Leonard, G.A]]
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[[Category: Round, E.S]]
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Revision as of 13:56, 16 November 2017

SAD structure of Thermolysin obtained by multi crystal data collection

5a3y, resolution 1.27Å

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