5d91
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum== | |
| + | <StructureSection load='5d91' size='340' side='right' caption='[[5d91]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5d91]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D91 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D91 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d92|5d92]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RSal33209_2010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d91 OCA], [http://pdbe.org/5d91 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d91 RCSB], [http://www.ebi.ac.uk/pdbsum/5d91 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d91 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Phosphatidylinositol is critical for intracellular signalling and anchoring of carbohydrates and proteins to outer cellular membranes. The defining step in phosphatidylinositol biosynthesis is catalysed by CDP-alcohol phosphotransferases, transmembrane enzymes that use CDP-diacylglycerol as donor substrate for this reaction, and either inositol in eukaryotes or inositol phosphate in prokaryotes as the acceptor alcohol. Here we report the structures of a related enzyme, the phosphatidylinositol-phosphate synthase from Renibacterium salmoninarum, with and without bound CDP-diacylglycerol to 3.6 and 2.5 A resolution, respectively. These structures reveal the location of the acceptor site, and the molecular determinants of substrate specificity and catalysis. Functional characterization of the 40%-identical ortholog from Mycobacterium tuberculosis, a potential target for the development of novel anti-tuberculosis drugs, supports the proposed mechanism of substrate binding and catalysis. This work therefore provides a structural and functional framework to understand the mechanism of phosphatidylinositol-phosphate biosynthesis. | ||
| - | + | Structural basis for phosphatidylinositol-phosphate biosynthesis.,Clarke OB, Tomasek D, Jorge CD, Dufrisne MB, Kim M, Banerjee S, Rajashankar KR, Shapiro L, Hendrickson WA, Santos H, Mancia F Nat Commun. 2015 Oct 16;6:8505. doi: 10.1038/ncomms9505. PMID:26510127<ref>PMID:26510127</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5d91" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Arcfu]] | ||
[[Category: Banerjee, S]] | [[Category: Banerjee, S]] | ||
| - | [[Category: | + | [[Category: Clarke, O B]] |
| - | [[Category: | + | [[Category: Dufrisne, M Belcher]] |
| - | [[Category: Hendrickson, W | + | [[Category: Hendrickson, W A]] |
| + | [[Category: Jorge, C D]] | ||
[[Category: Kim, M]] | [[Category: Kim, M]] | ||
[[Category: Mancia, F]] | [[Category: Mancia, F]] | ||
| - | [[Category: | + | [[Category: Rajashankar, K R]] |
| + | [[Category: Santos, H]] | ||
| + | [[Category: Tomasek, D T]] | ||
| + | [[Category: Enzyme]] | ||
| + | [[Category: Lipid biosynthesis]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Phosphatidylinositol]] | ||
Revision as of 14:05, 16 November 2017
Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum
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