2b7o
From Proteopedia
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|PDB= 2b7o |SIZE=350|CAPTION= <scene name='initialview01'>2b7o</scene>, resolution 2.30Å | |PDB= 2b7o |SIZE=350|CAPTION= <scene name='initialview01'>2b7o</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CE1:O-DODECANYL+OCTAETHYLENE+GLYCOL'>CE1</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Rv2178c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]) | |GENE= Rv2178c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b7o OCA], [http://www.ebi.ac.uk/pdbsum/2b7o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b7o RCSB]</span> | ||
}} | }} | ||
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[[Category: Webby, C J.]] | [[Category: Webby, C J.]] | ||
[[Category: XMTB, Mycobacterium Tuberculosis Structural Proteomics Project.]] | [[Category: XMTB, Mycobacterium Tuberculosis Structural Proteomics Project.]] | ||
| - | [[Category: CE1]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: PEP]] | ||
| - | [[Category: SO4]] | ||
[[Category: aromatic biosynthesis]] | [[Category: aromatic biosynthesis]] | ||
[[Category: dah7ps synthase]] | [[Category: dah7ps synthase]] | ||
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[[Category: xmtb]] | [[Category: xmtb]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:02:35 2008'' |
Revision as of 23:02, 30 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | Rv2178c (Mycobacterium tuberculosis) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Structure of 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase from Mycobacterium tuberculosis
Overview
The shikimate pathway, responsible for the biosynthesis of aromatic compounds, is essential for the growth of Mycobacterium tuberculosis and is a potential target for the design of new anti-tuberculosis drugs. The first step of this pathway is catalyzed by 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAH7PS). The DAH7PSs have been classified into two apparently unrelated types and, whereas structural data have been obtained for the type I DAH7PSs, no structural information is available for their type II counterparts. The type II DAH7PS from M.tuberculosis has been expressed in Escherichia coli, purified, functionally characterized and crystallized. It is found to be metal ion-dependent and subject to feedback inhibition by phenylalanine, tryptophan, tyrosine and chorismate, with a significant synergistic effect when tryptophan is used in combination with phenylalanine. The crystal structure of M.tuberculosis DAH7PS has been determined by single-wavelength anomalous diffraction and refined at 2.3A in complex with substrate phosphoenolpyruvate and Mn(2+). The structure reveals a tightly associated dimer of (beta/alpha)(8) TIM barrels. The monomer fold, the arrangement of key residues in the active site, and the binding modes of PEP and Mn(2+), all match those of the type I enzymes, and indicate a common ancestry for the type I and type II DAH7PSs, despite their minimal sequence identity. In contrast, the structural elements that decorate the core (beta/alpha)(8) fold differ from those in the type I enzymes, consistent with their different regulatory and oligomeric properties.
About this Structure
2B7O is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
The structure of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase from Mycobacterium tuberculosis reveals a common catalytic scaffold and ancestry for type I and type II enzymes., Webby CJ, Baker HM, Lott JS, Baker EN, Parker EJ, J Mol Biol. 2005 Dec 9;354(4):927-39. Epub 2005 Oct 21. PMID:16288916
Page seeded by OCA on Mon Mar 31 02:02:35 2008
Categories: Mycobacterium tuberculosis | Single protein | Baker, E N. | Baker, H M. | Lott, J S. | Parker, E J. | Webby, C J. | XMTB, Mycobacterium Tuberculosis Structural Proteomics Project. | Aromatic biosynthesis | Dah7ps synthase | Evolutionary relationship | Mycobacterium tuberculosis structural proteomics project | Shikimate pathway | Structural genomic | Xmtb
