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4xr1

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Revision as of 17:50, 16 November 2017

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- AG/AT mismatch.

Structural highlights

4xr1 is a 3 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1ecr, 2ewj, 2i05, 2i06, 4xr0, 4xr2, 4xr3
Gene:	tus, tau, b1610, JW1602 (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TUS_ECOLI] Trans-acting protein required for termination of DNA replication. Binds to DNA replication terminator sequences (terA to terF) to prevent the passage of replication forks. The termination efficiency will be affected by the affinity of this protein for the terminator sequence.

Publication Abstract from PubMed

In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites. Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction. However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes.

Replisome speed determines the efficiency of the Tus-Ter replication termination barrier.,Elshenawy MM, Jergic S, Xu ZQ, Sobhy MA, Takahashi M, Oakley AJ, Dixon NE, Hamdan SM Nature. 2015 Sep 17;525(7569):394-8. doi: 10.1038/nature14866. Epub 2015 Aug 31. PMID:26322585^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Elshenawy MM, Jergic S, Xu ZQ, Sobhy MA, Takahashi M, Oakley AJ, Dixon NE, Hamdan SM. Replisome speed determines the efficiency of the Tus-Ter replication termination barrier. Nature. 2015 Sep 17;525(7569):394-8. doi: 10.1038/nature14866. Epub 2015 Aug 31. PMID:26322585 doi:http://dx.doi.org/10.1038/nature14866

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xr1"

@@ Line 1: / Line 1: @@
 ==Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- AG/AT mismatch.==
 <StructureSection load='4xr1' size='340' side='right' caption='[[4xr1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4xr1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XR1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XR1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4xr1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XR1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XR1 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ecr|1ecr]], [[2ewj|2ewj]], [[2i05|2i05]], [[2i06|2i06]], [[4xr0|4xr0]], [[4xr2|4xr2]], [[4xr3|4xr3]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xr1 OCA], [http://pdbe.org/4xr1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xr1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xr1 PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tus, tau, b1610, JW1602 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xr1 OCA], [http://pdbe.org/4xr1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xr1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xr1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xr1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Ecoli]]
 [[Category: Oakley, A J]]
 [[Category: Dna complex]]

4xr1

From Proteopedia

Revision as of 17:50, 16 November 2017

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- AG/AT mismatch.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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