2be2
From Proteopedia
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|PDB= 2be2 |SIZE=350|CAPTION= <scene name='initialview01'>2be2</scene>, resolution 2.43Å | |PDB= 2be2 |SIZE=350|CAPTION= <scene name='initialview01'>2be2</scene>, resolution 2.43Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=R22:4-(3,5-DIMETHYLPHENOXY)-5-(FURAN-2-YLMETHYLSULFANYLMETHYL)-3-IODO-6-METHYLPYRIDIN-2(1H)-ONE'>R22</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span> |
|GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1]) | |GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2ban|2BAN]], [[2b5j|2B5J]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2be2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2be2 OCA], [http://www.ebi.ac.uk/pdbsum/2be2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2be2 RCSB]</span> | ||
}} | }} | ||
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[[Category: Oumouch, S.]] | [[Category: Oumouch, S.]] | ||
[[Category: Poncelet, A.]] | [[Category: Poncelet, A.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: R22]] | ||
| - | [[Category: SUC]] | ||
[[Category: aid]] | [[Category: aid]] | ||
[[Category: drug design]] | [[Category: drug design]] | ||
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[[Category: rt]] | [[Category: rt]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:04:58 2008'' |
Revision as of 23:05, 30 March 2008
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| , resolution 2.43Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | POL (Human immunodeficiency virus 1) | ||||||
| Activity: | RNA-directed DNA polymerase, with EC number 2.7.7.49 | ||||||
| Related: | 2BAN, 2B5J
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of HIV-1 reverse transcriptase (RT) in complex with R221239
Overview
In the treatment of AIDS, the efficacy of all drugs, including non-nucleoside inhibitors (NNRTIs) of HIV-1 reverse transcriptase (RT), has been limited by the rapid appearance of drug-resistant viruses. Lys103Asn, Tyr181Cys, and Tyr188Leu are some of the most common RT mutations that cause resistance to NNRTIs in the clinic. We report X-ray crystal structures for RT complexed with three different pyridinone derivatives, R157208, R165481, and R221239, at 2.95, 2.9, and 2.43 A resolution, respectively. All three ligands exhibit nanomolar or subnanomolar inhibitory activity against wild-type RT, but varying activities against drug-resistant mutants. R165481 and R221239 differ from most NNRTIs in that binding does not involve significant contacts with Tyr181. These compounds strongly inhibit wild-type HIV-1 RT and drug-resistant variants, including Tyr181Cys and Lys103Asn RT. These properties result in part from an iodine atom on the pyridinone ring of both inhibitors that interacts with the main-chain carbonyl oxygen of Tyr188. An acrylonitrile substituent on R165481 substantially improves the activity of the compound against wild-type RT (and several mutants) and provides a way to generate novel inhibitors that could interact with conserved elements of HIV-1 RT at the polymerase catalytic site. In R221239, there is a flexible linker to a furan ring that permits interactions with Val106, Phe227, and Pro236. These contacts appear to enhance the inhibitory activity of R221239 against the HIV-1 strains that carry the Val106Ala, Tyr188Leu, and Phe227Cys mutations.
About this Structure
2BE2 is a Protein complex structure of sequences from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.
Reference
Crystal structures for HIV-1 reverse transcriptase in complexes with three pyridinone derivatives: a new class of non-nucleoside inhibitors effective against a broad range of drug-resistant strains., Himmel DM, Das K, Clark AD Jr, Hughes SH, Benjahad A, Oumouch S, Guillemont J, Coupa S, Poncelet A, Csoka I, Meyer C, Andries K, Nguyen CH, Grierson DS, Arnold E, J Med Chem. 2005 Dec 1;48(24):7582-91. PMID:16302798
Page seeded by OCA on Mon Mar 31 02:04:58 2008
Categories: Human immunodeficiency virus 1 | Protein complex | RNA-directed DNA polymerase | Andries, K. | Arnold, E. | Benjahad, A. | Coupa, S. | Csoka, I. | Das, K. | Grierson, D S. | Guillemont, J. | Himmel, D M. | Hughes, S H. | Jr., A D.Clark. | Meyer, C. | Nguyen, C H. | Oumouch, S. | Poncelet, A. | Aid | Drug design | Drug resistance | Hiv | Protein-inhibitor complex | Reverse transcriptase | Rt
