4wyb

From Proteopedia

(Difference between revisions)

Revision as of 09:30, 22 November 2017

Structure of the Bud6 flank domain in complex with actin

Structural highlights

4wyb is a 24 chain structure with sequence from Baker's yeast and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	BUD6, AIP3, YLR319C, L8543.5 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [BUD6_YEAST] Not essential for mitotic growth but is necessary for normal morphogenesis. Involved in the organization and/or function of the actin cytoskeleton.

Publication Abstract from PubMed

In budding yeast, the actin-binding protein Bud6 cooperates with formins Bni1 and Bnr1 to catalyze the assembly of actin filaments. The nucleation-enhancing activity of Bud6 requires both a "core" domain that binds to the formin and a "flank" domain that binds monomeric actin. Here, we describe the structure of the Bud6 flank domain in complex with actin. Two helices in Bud6(flank) interact with actin; one binds in a groove at the barbed end of the actin monomer in a manner closely resembling the helix of WH2 domains, a motif found in many actin nucleation factors. The second helix rises along the face of actin. Mutational analysis verifies the importance of these Bud6-actin contacts for nucleation-enhancing activity. The Bud6 binding site on actin overlaps with that of the formin FH2 domain and is also incompatible with inter-subunit contacts in F-actin, suggesting that Bud6 interacts only transiently with actin monomers during filament nucleation.

Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif.,Park E, Graziano BR, Zheng W, Garabedian M, Goode BL, Eck MJ Structure. 2015 Aug 4;23(8):1492-9. doi: 10.1016/j.str.2015.05.015. Epub 2015 Jun, 25. PMID:26118535^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Park E, Graziano BR, Zheng W, Garabedian M, Goode BL, Eck MJ. Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif. Structure. 2015 Aug 4;23(8):1492-9. doi: 10.1016/j.str.2015.05.015. Epub 2015 Jun, 25. PMID:26118535 doi:http://dx.doi.org/10.1016/j.str.2015.05.015

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wyb"

@@ Line 3: / Line 3: @@
 <StructureSection load='4wyb' size='340' side='right' caption='[[4wyb]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4wyb]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4wyb]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYB FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyb OCA], [http://pdbe.org/4wyb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wyb RCSB], [http://www.ebi.ac.uk/pdbsum/4wyb PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BUD6, AIP3, YLR319C, L8543.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyb OCA], [http://pdbe.org/4wyb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wyb RCSB], [http://www.ebi.ac.uk/pdbsum/4wyb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyb ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
 [[Category: Oryctolagus cuniculus]]
 [[Category: Eck, M J]]

4wyb

From Proteopedia

Revision as of 09:30, 22 November 2017

Structure of the Bud6 flank domain in complex with actin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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