2bjv
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjv OCA], [http://www.ebi.ac.uk/pdbsum/2bjv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bjv RCSB]</span> | ||
}} | }} | ||
| Line 32: | Line 35: | ||
[[Category: Wigneshweraraj, S.]] | [[Category: Wigneshweraraj, S.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
| - | [[Category: aaa]] | + | [[Category: aaa,transcription activation]] |
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: dna-binding]] | [[Category: dna-binding]] | ||
| Line 39: | Line 42: | ||
[[Category: pspf]] | [[Category: pspf]] | ||
[[Category: sigma54 activator]] | [[Category: sigma54 activator]] | ||
| - | + | [[Category: transcription regulation,]] | |
| - | [[Category: transcription regulation]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:29 2008'' |
Revision as of 23:07, 30 March 2008
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| , resolution 1.70Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT
Overview
Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
About this Structure
2BJV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859
Page seeded by OCA on Mon Mar 31 02:07:29 2008
Categories: Escherichia coli | Single protein | Beuron, F. | Bordes, P. | Buck, M. | Keetch, C A. | Niwa, H. | Rappas, M. | Robinson, C V. | Schumacher, J. | Wigneshweraraj, S. | Zhang, X. | Aaa,transcription activation | Atp-binding | Dna-binding | Enhancer binding protein | Gene regulation | Pspf | Sigma54 activator | Transcription regulation,
