2bkj
From Proteopedia
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|PDB= 2bkj |SIZE=350|CAPTION= <scene name='initialview01'>2bkj</scene>, resolution 2.08Å | |PDB= 2bkj |SIZE=350|CAPTION= <scene name='initialview01'>2bkj</scene>, resolution 2.08Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene> | + | |LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/FMN_reductase FMN reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.29 1.5.1.29] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase FMN reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.29 1.5.1.29] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bkj OCA], [http://www.ebi.ac.uk/pdbsum/2bkj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bkj RCSB]</span> | ||
}} | }} | ||
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[[Category: TU, S C.]] | [[Category: TU, S C.]] | ||
[[Category: Tanner, J J.]] | [[Category: Tanner, J J.]] | ||
| - | [[Category: FMN]] | ||
| - | [[Category: NAD]] | ||
[[Category: frp]] | [[Category: frp]] | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:45 2008'' |
Revision as of 23:07, 30 March 2008
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| , resolution 2.08Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | FMN reductase, with EC number 1.5.1.29 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI COMPLEXED WITH NAD+
Overview
The 2.1 A resolution crystal structure of flavin reductase P with the inhibitor nicotinamide adenine dinucleotide (NAD) bound in the active site has been determined. NAD adopts a novel, folded conformation in which the nicotinamide and adenine rings stack in parallel with an inter-ring distance of 3.6 A. The pyrophosphate binds next to the flavin cofactor isoalloxazine, while the stacked nicotinamide/adenine moiety faces away from the flavin. The observed NAD conformation is quite different from the extended conformations observed in other enzyme/NAD(P) structures; however, it resembles the conformation proposed for NAD in solution. The flavin reductase P/NAD structure provides new information about the conformational diversity of NAD, which is important for understanding catalysis. This structure offers the first crystallographic evidence of a folded NAD with ring stacking, and it is the first enzyme structure containing an FMN cofactor interacting with NAD(P). Analysis of the structure suggests a possible dynamic mechanism underlying NADPH substrate specificity and product release that involves unfolding and folding of NADP(H).
About this Structure
2BKJ is a Single protein structure of sequence from Vibrio harveyi. Full crystallographic information is available from OCA.
Reference
Unusual folded conformation of nicotinamide adenine dinucleotide bound to flavin reductase P., Tanner JJ, Tu SC, Barbour LJ, Barnes CL, Krause KL, Protein Sci. 1999 Sep;8(9):1725-32. PMID:10493573
Page seeded by OCA on Mon Mar 31 02:07:45 2008
