5uyt
From Proteopedia
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<StructureSection load='5uyt' size='340' side='right' caption='[[5uyt]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='5uyt' size='340' side='right' caption='[[5uyt]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5uyt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UYT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5uyt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Flab3 Flab3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UYT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UYT FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FIC_00493 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=531844 FLAB3])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uyt OCA], [http://pdbe.org/5uyt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uyt RCSB], [http://www.ebi.ac.uk/pdbsum/5uyt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uyt ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uyt OCA], [http://pdbe.org/5uyt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uyt RCSB], [http://www.ebi.ac.uk/pdbsum/5uyt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uyt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Antifreeze proteins (AFPs) enhance the survival of organisms inhabiting cold environments by affecting the formation and/or structure of ice. We report the crystal structure of the first multi-domain AFP that has been characterized. The two ice binding domains are structurally similar. Each consists of an irregular beta-helix with a triangular cross-section and a long alpha-helix that runs parallel on one side of the beta-helix. Both domains are stabilized by hydrophobic interactions. A flat plane on the same face of each domain's beta-helix was identified as the ice binding site. Mutating any of the smaller residues on the ice binding site to bulkier ones decreased the antifreeze activity. The bulky side chain of Leu174 in domain A sterically hinders the binding of water molecules to the protein backbone, partially explaining why antifreeze activity by domain A is inferior to that of domain B. Our data provide a molecular basis for understanding differences in antifreeze activity between the two domains of this protein and general insight on how structural differences in the ice-binding sites affect the activity of AFPs. | ||
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| + | Structural basis of antifreeze activity of a bacterial multi-domain antifreeze protein.,Wang C, Pakhomova S, Newcomer ME, Christner BC, Luo BH PLoS One. 2017 Nov 6;12(11):e0187169. doi: 10.1371/journal.pone.0187169., eCollection 2017. PMID:29108002<ref>PMID:29108002</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5uyt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Flab3]] | ||
[[Category: Christner, B C]] | [[Category: Christner, B C]] | ||
[[Category: Luo, B H]] | [[Category: Luo, B H]] | ||
Current revision
Crystal structure of ice binding protein from an Antarctic bacterium Flavobacteriaceae
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