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5w3x

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Revision as of 10:32, 22 November 2017

Crystal structure of PopP2 in complex with IP6, AcCoA and the WRKY domain of RRS1-R .

Structural highlights

5w3x is a 4 chain structure with sequence from "bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896 and Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	5w40, 5w3y, 5w3t
Gene:	popp, RUN1985_v1_1190012 ("Bacillus solanacearum" Smith 1896), RRS1, RCH2, RRS1-R, WRKY52 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[WR52W_ARATH] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Acts also as a disease resistance protein involved in resistance to fungal and bacterial pathogens, including R.solanacearum, P.syringae pv. tomato and C.higginsianum. In presence of RPS4, elicites an EDS1-dependent hypersensitive response (PubMed:24146667).[UniProtKB:Q9FH83]^[1] ^[2] ^[3]

Publication Abstract from PubMed

The Yersinia outer protein J (YopJ) family of bacterial effectors depends on a novel acetyltransferase domain to acetylate signalling proteins from plant and animal hosts. However, the underlying mechanism is unclear. Here, we report the crystal structures of PopP2, a YopJ effector produced by the plant pathogen Ralstonia solanacearum, in complex with inositol hexaphosphate (InsP6), acetyl-coenzyme A (AcCoA) and/or substrate Resistance to Ralstonia solanacearum 1 (RRS1-R)WRKY. PopP2 recognizes the WRKYGQK motif of RRS1-RWRKY to position a targeted lysine in the active site for acetylation. Importantly, the PopP2-RRS1-RWRKY association is allosterically regulated by InsP6 binding, suggesting a previously unidentified role of the eukaryote-specific cofactor in substrate interaction. Furthermore, we provide evidence for the reaction intermediate of PopP2-mediated acetylation, an acetyl-cysteine covalent adduct, lending direct support to the 'ping-pong'-like catalytic mechanism proposed for YopJ effectors. Our study provides critical mechanistic insights into the virulence activity of YopJ class of acetyltransferases.

Mechanism of host substrate acetylation by a YopJ family effector.,Zhang ZM, Ma KW, Gao L, Hu Z, Schwizer S, Ma W, Song J Nat Plants. 2017 Jul 24;3:17115. doi: 10.1038/nplants.2017.115. PMID:28737762^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Narusaka M, Shirasu K, Noutoshi Y, Kubo Y, Shiraishi T, Iwabuchi M, Narusaka Y. RRS1 and RPS4 provide a dual Resistance-gene system against fungal and bacterial pathogens. Plant J. 2009 Oct;60(2):218-26. doi: 10.1111/j.1365-313X.2009.03949.x. Epub 2009 , Jun 9. PMID:19519800 doi:http://dx.doi.org/10.1111/j.1365-313X.2009.03949.x
↑ Narusaka M, Kubo Y, Shiraishi T, Iwabuchi M, Narusaka Y. A dual resistance gene system prevents infection by three distinct pathogens. Plant Signal Behav. 2009 Oct;4(10):954-5. Epub 2009 Oct 17. PMID:19826224
↑ Heidrich K, Tsuda K, Blanvillain-Baufume S, Wirthmueller L, Bautor J, Parker JE. Arabidopsis TNL-WRKY domain receptor RRS1 contributes to temperature-conditioned RPS4 auto-immunity. Front Plant Sci. 2013 Oct 17;4:403. doi: 10.3389/fpls.2013.00403. eCollection, 2013. PMID:24146667 doi:http://dx.doi.org/10.3389/fpls.2013.00403
↑ Zhang ZM, Ma KW, Gao L, Hu Z, Schwizer S, Ma W, Song J. Mechanism of host substrate acetylation by a YopJ family effector. Nat Plants. 2017 Jul 24;3:17115. doi: 10.1038/nplants.2017.115. PMID:28737762 doi:http://dx.doi.org/10.1038/nplants.2017.115

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5w3x"

@@ Line 3: / Line 3: @@
 <StructureSection load='5w3x' size='340' side='right' caption='[[5w3x]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896] and [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3X FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w40|5w40]], [[5w3y|5w3y]], [[5w3t|5w3t]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">popp, RUN1985_v1_1190012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896]), RRS1, RCH2, RRS1-R, WRKY52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [http://pdbe.org/5w3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [http://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>
 </table>
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Bacillus solanacearum smith 1896]]
+[[Category: Arath]]
 [[Category: Gao, L]]
 [[Category: Song, J]]

5w3x

From Proteopedia

Revision as of 10:32, 22 November 2017

Crystal structure of PopP2 in complex with IP6, AcCoA and the WRKY domain of RRS1-R .

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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