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1b9l

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Revision as of 11:18, 22 November 2017

7,8-DIHYDRONEOPTERIN TRIPHOSPHATE EPIMERASE

Structural highlights

1b9l is a 8 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FOLX_ECOLI] Catalyzes the epimerization of carbon 2' of the side chain of dihydroneopterin triphosphate (H2NTP) to form dihydromonapterin triphosphate (H2MTP).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

BACKGROUND: Dihydroneopterin triphosphate (H2NTP) is the central substrate in the biosynthesis of folate and tetrahydrobiopterin. Folate serves as a cofactor in amino acid and purine biosynthesis and tetrahydrobiopterin is used as a cofactor in amino acid hydroxylation and nitric oxide synthesis. In bacteria, H2NTP enters the folate biosynthetic pathway after nonenzymatic dephosphorylation; in vertebrates, H2NTP is used to synthesize tetrahydrobiopterin. The dihydroneopterin triphosphate epimerase of Escherichia coli catalyzes the inversion of carbon 2' of H2NTP. RESULTS: The crystal structure of the homo-octameric protein has been solved by a combination of multiple isomorphous replacement, Patterson search techniques and cyclic averaging and has been refined to a crystallographic R factor of 18.8% at 2.9 A resolution. The enzyme is a torus-shaped, D4 symmetric homo-octamer with approximate dimensions of 65 x 65 A. Four epimerase monomers form an unusual 16-stranded antiparallel beta barrel by tight association between the N- and C-terminal beta strands of two adjacent subunits. Two tetramers associate in a head-to-head fashion to form the active enzyme complex. CONCLUSIONS: The folding topology, quaternary structure and amino acid sequence of epimerase is similar to that of the dihydroneopterin aldolase involved in the biosynthesis of the vitamin folic acid. The monomer fold of epimerase is also topologically similar to that of GTP cyclohydrolase I (GTP CH-1), 6-pyrovoyl tetrahydropterin synthase (PTPS) and uroate oxidase (UO). Despite a lack of significant sequence homology these proteins share a common subunit fold and oligomerize to form central beta barrel structures employing different cyclic symmetry elements, D4, D5, D3 and D2, respectively. Moreover, these enzymes have a topologically equivalent acceptor site for the 2-amino-4-oxo pyrimidine (2-oxo-4-oxo pyrimidine in uroate oxidase) moiety of their respective substrates.

Crystal structure of 7,8-dihydroneopterin triphosphate epimerase.,Ploom T, Haussmann C, Hof P, Steinbacher S, Bacher A, Richardson J, Huber R Structure. 1999 May;7(5):509-16. PMID:10378270^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ploom T, Haussmann C, Hof P, Steinbacher S, Bacher A, Richardson J, Huber R. Crystal structure of 7,8-dihydroneopterin triphosphate epimerase. Structure. 1999 May;7(5):509-16. PMID:10378270

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1b9l"

@@ Line 1: / Line 1: @@
 ==7,8-DIHYDRONEOPTERIN TRIPHOSPHATE EPIMERASE==
 <StructureSection load='1b9l' size='340' side='right' caption='[[1b9l]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1b9l]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1B9L FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b9l OCA], [http://pdbe.org/1b9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b9l RCSB], [http://www.ebi.ac.uk/pdbsum/1b9l PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b9l OCA], [http://pdbe.org/1b9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1b9l RCSB], [http://www.ebi.ac.uk/pdbsum/1b9l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1b9l ProSAT]</span></td></tr>
 </table>
 == Function ==

1b9l

From Proteopedia

Revision as of 11:18, 22 November 2017

7,8-DIHYDRONEOPTERIN TRIPHOSPHATE EPIMERASE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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