2bpl

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|PDB= 2bpl |SIZE=350|CAPTION= <scene name='initialview01'>2bpl</scene>, resolution 2.05&Aring;
|PDB= 2bpl |SIZE=350|CAPTION= <scene name='initialview01'>2bpl</scene>, resolution 2.05&Aring;
|SITE= <scene name='pdbsite=AC1:F6r+Binding+Site+For+Chain+B'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:F6r+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=F6R:FRUCTOSE -6-PHOSPHATE'>F6R</scene>
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|LIGAND= <scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bpl OCA], [http://www.ebi.ac.uk/pdbsum/2bpl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bpl RCSB]</span>
}}
}}
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[[Category: Golinelli-Pimpaneau, B.]]
[[Category: Golinelli-Pimpaneau, B.]]
[[Category: Mouilleron, S.]]
[[Category: Mouilleron, S.]]
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[[Category: F6R]]
 
[[Category: amidotransferase]]
[[Category: amidotransferase]]
[[Category: ammonia channeling]]
[[Category: ammonia channeling]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:04:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:09:51 2008''

Revision as of 23:09, 30 March 2008

Image:2bpl.gif


PDB ID 2bpl

Drag the structure with the mouse to rotate
, resolution 2.05Å
Sites:
Ligands:
Activity: Glutamine--fructose-6-phosphate transaminase (isomerizing), with EC number 2.6.1.16
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



E.COLI GLUCOSAMINE-6P SYNTHASE IN COMPLEX WITH FRUCTOSE-6P


Overview

Glucosamine-6P synthase catalyzes the synthesis of glucosamine-6P from fructose-6P and glutamine and uses a channel to transfer ammonia from its glutaminase to its synthase active site. X-ray structures of glucosamine-6P synthase have been determined at 2.05 Angstroms resolution in the presence of fructose-6P and at 2.35 Angstroms resolution in the presence of fructose-6P and 6-diazo-5-oxo-L-norleucine, a glutamine affinity analog that covalently modifies the N-terminal catalytic cysteine, therefore mimicking the gamma-glutamyl-thioester intermediate formed during hydrolysis of glutamine. The fixation of the glutamine analog activates the enzyme through several major structural changes: 1) the closure of a loop to shield the glutaminase site accompanied by significant domain hinging, 2) the activation of catalytic residues involved in glutamine hydrolysis, i.e. the alpha-amino group of Cys-1 and Asn-98 that is positioned to form the oxyanion hole, and 3) a 75 degrees rotation of the Trp-74 indole group that opens the ammonia channel.

About this Structure

2BPL is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:16339762

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