2bwf
From Proteopedia
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|PDB= 2bwf |SIZE=350|CAPTION= <scene name='initialview01'>2bwf</scene>, resolution 1.15Å | |PDB= 2bwf |SIZE=350|CAPTION= <scene name='initialview01'>2bwf</scene>, resolution 1.15Å | ||
|SITE= <scene name='pdbsite=AC1:Fmt+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Fmt+Binding+Site+For+Chain+A'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene> | + | |LIGAND= <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bwf OCA], [http://www.ebi.ac.uk/pdbsum/2bwf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bwf RCSB]</span> | ||
}} | }} | ||
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[[Category: Noble, M E.M.]] | [[Category: Noble, M E.M.]] | ||
[[Category: Trempe, J-F.]] | [[Category: Trempe, J-F.]] | ||
| - | [[Category: FMT]] | ||
[[Category: signaling protein]] | [[Category: signaling protein]] | ||
[[Category: uba]] | [[Category: uba]] | ||
[[Category: ubiquitin]] | [[Category: ubiquitin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:12:48 2008'' |
Revision as of 23:12, 30 March 2008
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| , resolution 1.15Å | |||||||
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| Sites: | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STURCTURE OF THE UBL DOMAIN OF DSK2 FROM S. CEREVISIAE
Overview
The yeast proteins Dsk2 and Rad23 belong to a family of proteins that contain an N-terminal ubiquitin-like domain (UBL) and a C-terminal ubiquitin-associated domain (UBA). Both Dsk2 and Rad23 function as adaptors to target ubiquitin-labelled proteins to the proteasome through recognition of polyubiquitin (four or more K48-linked ubiquitins) by their UBA domains and to the yeast proteasomal subunit Rpn1 by their UBL domains. The crystal structures of the Dsk2 UBL domain, the Dsk2 UBA domain and the Dsk2 UBA-UBL complex are reported. In the crystal, the Dsk2 UBA domains associate through electrostatic interactions to form ninefold helical ribbons that leave the ubiquitin-binding surface exposed. The UBA-UBL complex explains the reduced affinity of the UBA domain for UBL compared with ubiquitin and has implications for the regulation of Dsk2 adaptor function during ubiquitin-mediated proteasomal targeting. A model is discussed in which two or more Dsk2 UBA molecules may selectively bind to K48-linked polyubiquitin.
About this Structure
2BWF is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structures of the Dsk2 UBL and UBA domains and their complex., Lowe ED, Hasan N, Trempe JF, Fonso L, Noble ME, Endicott JA, Johnson LN, Brown NR, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):177-88. Epub 2006, Jan 18. PMID:16421449
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