2by7

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|PDB= 2by7 |SIZE=350|CAPTION= <scene name='initialview01'>2by7</scene>, resolution 1.30&Aring;
|PDB= 2by7 |SIZE=350|CAPTION= <scene name='initialview01'>2by7</scene>, resolution 1.30&Aring;
|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+X'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+X'>AC1</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|LIGAND= <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2by7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2by7 OCA], [http://www.ebi.ac.uk/pdbsum/2by7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2by7 RCSB]</span>
}}
}}
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[[Category: Ravelli, R B.G.]]
[[Category: Ravelli, R B.G.]]
[[Category: Timmins, J.]]
[[Category: Timmins, J.]]
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[[Category: BAM]]
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[[Category: data collection,radiation damage,dose-rate,synchrotron radiation,hydrolase]]
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[[Category: CA]]
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[[Category: GOL]]
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[[Category: SO4]]
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[[Category: data collection]]
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[[Category: dose-rate]]
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[[Category: hydrolase]]
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[[Category: radiation damage]]
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[[Category: synchrotron radiation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:08:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:13:33 2008''

Revision as of 23:13, 30 March 2008

Image:2by7.gif


PDB ID 2by7

Drag the structure with the mouse to rotate
, resolution 1.30Å
Sites:
Ligands: , , ,
Activity: Trypsin, with EC number 3.4.21.4
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION


Overview

This paper focuses on the radiation-damage effects when applying the same total X-ray dose to protein crystals at different dose rates. These experiments have been performed on both a selenomethionated protein and on bovine trypsin using dose rates that span nearly two orders of magnitude. The results show no clear dose-rate effect on the global indicators of radiation damage, but a small measurable dose-rate effect could be found when studying specific radiation damage. It is hypothesized that this observed dose-rate effect relates to differences in the steady-state free-radical concentration.

About this Structure

2BY7 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442

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