2c1w
From Proteopedia
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|PDB= 2c1w |SIZE=350|CAPTION= <scene name='initialview01'>2c1w</scene>, resolution 2.20Å | |PDB= 2c1w |SIZE=350|CAPTION= <scene name='initialview01'>2c1w</scene>, resolution 2.20Å | ||
|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+C'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+C'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c1w OCA], [http://www.ebi.ac.uk/pdbsum/2c1w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c1w RCSB]</span> | ||
}} | }} | ||
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[[Category: Renzi, F.]] | [[Category: Renzi, F.]] | ||
[[Category: Vallone, B.]] | [[Category: Vallone, B.]] | ||
| - | [[Category: PO4]] | ||
[[Category: endoribonuclease]] | [[Category: endoribonuclease]] | ||
[[Category: nuclease]] | [[Category: nuclease]] | ||
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[[Category: splicing independent processing]] | [[Category: splicing independent processing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:15:06 2008'' |
Revision as of 23:15, 30 March 2008
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| , resolution 2.20Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE OF XENDOU: A SPLICING INDEPENDENT SNORNA PROCESSING ENDORIBONUCLEASE
Overview
Small nucleolar RNAs (snoRNAs) play a key role in eukaryotic ribosome biogenesis. In most cases, snoRNAs are encoded in introns and are released through the splicing reaction. Some snoRNAs are, instead, produced by an alternative pathway consisting of endonucleolytic processing of pre-mRNA. XendoU, the endoribonuclease responsible for this activity, is a U-specific, metal-dependent enzyme that releases products with 2'-3' cyclic phosphate termini. XendoU is broadly conserved among eukaryotes, and it is a genetic marker of nidoviruses, including the severe acute respiratory syndrome coronavirus, where it is essential for replication and transcription. We have determined by crystallography the structure of XendoU that, by refined search methodologies, appears to display a unique fold. Based on sequence conservation, mutagenesis, and docking simulations, we have identified the active site. The conserved structural determinants of this site may provide a framework for attempting to design antiviral drugs to interfere with the infectious nidovirus life cycle.
About this Structure
2C1W is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
The structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication., Renzi F, Caffarelli E, Laneve P, Bozzoni I, Brunori M, Vallone B, Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12365-70. Epub 2006 Aug 8. PMID:16895992
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