5xut
From Proteopedia
(Difference between revisions)
m (Protected "5xut" [edit=sysop:move=sysop]) |
|||
| Line 3: | Line 3: | ||
<StructureSection load='5xut' size='340' side='right' caption='[[5xut]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='5xut' size='340' side='right' caption='[[5xut]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5xut]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XUT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XUT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xut]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_nd2006 Lachnospiraceae bacterium nd2006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XUT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XUT FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xut OCA], [http://pdbe.org/5xut PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xut RCSB], [http://www.ebi.ac.uk/pdbsum/5xut PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xut ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xut OCA], [http://pdbe.org/5xut PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xut RCSB], [http://www.ebi.ac.uk/pdbsum/5xut PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xut ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic genome editing. Cpf1 requires a specific nucleotide sequence, called a protospacer adjacent motif (PAM), for target recognition. Besides the canonical TTTV PAM, Cpf1 recognizes suboptimal C-containing PAMs. Here, we report four crystal structures of LbCpf1 in complex with the crRNA and its target DNA containing either TTTA, TCTA, TCCA, or CCCA as the PAM. These structures revealed that, depending on the PAM sequences, LbCpf1 undergoes conformational changes to form altered interactions with the PAM-containing DNA duplexes, thereby achieving the relaxed PAM recognition. Collectively, the present structures advance our mechanistic understanding of the PAM-dependent, crRNA-guided DNA cleavage by the Cpf1 family nucleases. | ||
| + | |||
| + | Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1.,Yamano T, Zetsche B, Ishitani R, Zhang F, Nishimasu H, Nureki O Mol Cell. 2017 Aug 17;67(4):633-645.e3. doi: 10.1016/j.molcel.2017.06.035. Epub, 2017 Aug 3. PMID:28781234<ref>PMID:28781234</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5xut" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Lachnospiraceae bacterium nd2006]] | ||
[[Category: Ishitani, R]] | [[Category: Ishitani, R]] | ||
[[Category: Nishimasu, H]] | [[Category: Nishimasu, H]] | ||
Revision as of 07:57, 6 December 2017
Crystal structure of Lachnospiraceae bacterium ND2006 Cpf1 in complex with crRNA and target DNA (TCTA PAM)
| |||||||||||
