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5vlz

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Revision as of 08:03, 6 December 2017

Warning: this is a large structure, and loading might take a long time or not happen at all.

Backbone model for phage Qbeta capsid

Structural highlights

5vlz is a 181 chain structure with sequence from Allolevivirus subgroup iii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5vly, 5vm7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Warning: this is a large structure, and loading might take a long time or not happen at all.

Function

[CAPSD_BPQBE] Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 26 nm in diameter, and consisting of 89 capsid proteins dimers (178 capsid proteins) (PubMed:27671640, PubMed:19913556). Involved in viral genome encapsidation through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome (PubMed:8943226, PubMed:27671640). Binding of the capsid proteins to the viral RNA induces a conformational change required for efficient T=3 shell formation (PubMed:19913556). The capsid contains also 1 copy of the A2 maturation protein (PubMed:27671640).^[1] ^[2] ^[3] Acts as a translational repressor of viral replicase synthesis late in infection. This latter function is the result of capsid protein interaction with an RNA hairpin which contains the replicase ribosome-binding site.^[4] [MATA2_BPQBE] Induces host cell lysis (PubMed:11892805). Inhibits host MurA activity thereby blocking the synthesis of murein precursors necessary for the host cell wall biosynthesis (PubMed:11423662). May be responsible for the attachment to the host pilus. Makes extensive contacts with the viral genome (PubMed:28111107).^[5] ^[6] ^[7] ^[8]

References

↑ Basnak G, Morton VL, Rolfsson O, Stonehouse NJ, Ashcroft AE, Stockley PG. Viral genomic single-stranded RNA directs the pathway toward a T=3 capsid. J Mol Biol. 2010 Feb 5;395(5):924-36. doi: 10.1016/j.jmb.2009.11.018. Epub 2009, Nov 12. PMID:19913556 doi:http://dx.doi.org/10.1016/j.jmb.2009.11.018
↑ Gorzelnik KV, Cui Z, Reed CA, Jakana J, Young R, Zhang J. Asymmetric cryo-EM structure of the canonical Allolevivirus Qbeta reveals a single maturation protein and the genomic ssRNA in situ. Proc Natl Acad Sci U S A. 2016 Sep 26. pii: 201609482. PMID:27671640 doi:http://dx.doi.org/10.1073/pnas.1609482113
↑ Lim F, Spingola M, Peabody DS. The RNA-binding site of bacteriophage Qbeta coat protein. J Biol Chem. 1996 Dec 13;271(50):31839-45. PMID:8943226
↑ Lim F, Spingola M, Peabody DS. The RNA-binding site of bacteriophage Qbeta coat protein. J Biol Chem. 1996 Dec 13;271(50):31839-45. PMID:8943226
↑ Bernhardt TG, Wang IN, Struck DK, Young R. A protein antibiotic in the phage Qbeta virion: diversity in lysis targets. Science. 2001 Jun 22;292(5525):2326-9. PMID:11423662 doi:http://dx.doi.org/10.1126/science.1058289
↑ Karnik S, Billeter M. The lysis function of RNA bacteriophage Qbeta is mediated by the maturation (A2) protein. EMBO J. 1983;2(9):1521-6. PMID:11892805
↑ Reed CA, Langlais C, Wang IN, Young R. A(2) expression and assembly regulates lysis in Qbeta infections. Microbiology. 2013 Mar;159(Pt 3):507-14. doi: 10.1099/mic.0.064790-0. Epub 2013, Jan 17. PMID:23329676 doi:http://dx.doi.org/10.1099/mic.0.064790-0
↑ Rumnieks J, Tars K. Crystal Structure of the Maturation Protein from Bacteriophage Qbeta. J Mol Biol. 2017 Jan 19. pii: S0022-2836(17)30040-2. doi:, 10.1016/j.jmb.2017.01.012. PMID:28111107 doi:http://dx.doi.org/10.1016/j.jmb.2017.01.012

1 Structural highlights
2 Function
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5vlz"

@@ Line 3: / Line 3: @@
 <StructureSection load='5vlz' size='340' side='right' caption='[[5vlz]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5vlz]] is a 181 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_phage_qbeta Escherichia phage qbeta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5vlz]] is a 181 chain structure with sequence from [http://en.wikipedia.org/wiki/Allolevivirus_subgroup_iii Allolevivirus subgroup iii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLZ FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vly|5vly]], [[5vm7|5vm7]]</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlz OCA], [http://pdbe.org/5vlz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlz RCSB], [http://www.ebi.ac.uk/pdbsum/5vlz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlz ProSAT]</span></td></tr>
@@ Line 14: / Line 14: @@
 __TOC__
 </StructureSection>
-[[Category: Escherichia phage qbeta]]
+[[Category: Allolevivirus subgroup iii]]
 [[Category: Cui, Z]]
 [[Category: Zhang, J]]

5vlz

From Proteopedia

Revision as of 08:03, 6 December 2017

Backbone model for phage Qbeta capsid

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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