2c8o
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8o OCA], [http://www.ebi.ac.uk/pdbsum/2c8o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c8o RCSB]</span> | ||
}} | }} | ||
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[[Category: visualisation]] | [[Category: visualisation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:17:57 2008'' |
Revision as of 23:17, 30 March 2008
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| , resolution 1.50Å | |||||||
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| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
LYSOZYME (1SEC) AND UV LASR EXCITED FLUORESCENCE
Overview
Structural proteomics has promoted the rapid development of automated protein structure determination using X-ray crystallography. Robotics are now routinely used along the pipeline from genes to protein structures. However, a bottleneck still remains. At synchrotron beamlines, the success rate of automated sample alignment along the X-ray beam is limited by difficulties in visualization of protein crystals, especially when they are small and embedded in mother liquor. Despite considerable improvement in optical microscopes, the use of visible light transmitted or reflected by the sample may result in poor or misleading contrast. Here, the endogenous fluorescence from aromatic amino acids has been used to identify even tiny or weakly fluorescent crystals with a high success rate. The use of a compact laser at 266 nm in combination with non-fluorescent sample holders provides an efficient solution to collect high-contrast fluorescence images in a few milliseconds and using standard camera optics. The best image quality was obtained with direct illumination through a viewing system coaxial with the UV beam. Crystallographic data suggest that the employed UV exposures do not generate detectable structural damage.
About this Structure
2C8O is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops., Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):253-61. Epub 2006, Feb 22. PMID:16510972
Page seeded by OCA on Mon Mar 31 02:17:57 2008
Categories: Lysozyme | Single protein | Bourgeois, D. | Cipriani, F. | Felisaz, F. | Jacquamet, L. | Joly, J. | Lavault, B. | Nurizzo, D. | Ohana, J. | Vernede, X. | Allergen | Antimicrobial | Bacteriolytic enzyme | Hydrolase | Laser | Uv | Visualisation
