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5gpr
From Proteopedia
(Difference between revisions)
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<StructureSection load='5gpr' size='340' side='right' caption='[[5gpr]], [[Resolution|resolution]] 3.23Å' scene=''> | <StructureSection load='5gpr' size='340' side='right' caption='[[5gpr]], [[Resolution|resolution]] 3.23Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5gpr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GPR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asian_corn_borer Asian corn borer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GPR FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OfChi-h ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93504 Asian corn borer])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpr OCA], [http://pdbe.org/5gpr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpr RCSB], [http://www.ebi.ac.uk/pdbsum/5gpr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpr ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpr OCA], [http://pdbe.org/5gpr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpr RCSB], [http://www.ebi.ac.uk/pdbsum/5gpr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chitinase-h (Chi-h) is of special interest among insect chitinases due to its exclusive distribution in lepidopteran insects and high sequence identity with bacterial and baculovirus homologs. Here OfChi-h, a Chi-h from Ostrinia furnacalis, was investigated. Crystal structures of both OfChi-h and its complex with chitoheptaose ((GlcN)7) reveal that OfChi-h possesses a long and asymmetric substrate binding cleft, which is a typical characteristics of a processive exo-chitinase. The structural comparison between OfChi-h and its bacterial homolog SmChiA uncovered two phenylalanine-to-tryptophan site variants in OfChi-h at subsites +2 and possibly -7. The F232W/F396W double mutant endowed SmChiA with higher hydrolytic activities toward insoluble substrates, such as insect cuticle, alpha-chitin, and chitin nanowhisker. An enzymatic assay demonstrated that OfChi-h outperformed OfChtI, an insect endo-chitinase, toward the insoluble substrates, but showed lower activity toward the soluble substrate ethylene glycol chitin. Furthermore, OfChi-h was found to be inhibited by N,N',N''-trimethylglucosamine-N,N',N'',N'''-tetraacetylchitotetraose (TMG-(GlcNAc)4), a substrate analog which can be degraded into TMG-(GlcNAc)1-2 Injection of TMG-(GlcNAc)4 into 5th-instar O. furnacalis larvae led to severe defects in pupation. This work provides insights into a molting-indispensable insect chitinase that is phylogenetically closer to bacterial chitinases than insect chitinases. | ||
| + | |||
| + | Structure, Catalysis, and Inhibition of OfChi-h, the Lepidoptera-exclusive Insect Chitinase.,Liu T, Chen L, Zhou Y, Jiang X, Duan Y, Yang Q J Biol Chem. 2017 Feb 10;292(6):2080-2088. doi: 10.1074/jbc.M116.755330. Epub, 2017 Jan 4. PMID:28053084<ref>PMID:28053084</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5gpr" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Asian corn borer]] | ||
[[Category: Chen, L]] | [[Category: Chen, L]] | ||
[[Category: Liu, T]] | [[Category: Liu, T]] | ||
Revision as of 07:22, 13 December 2017
Crystal structure of chitinase-h from Ostrinia furnacalis
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