1e8l

From Proteopedia

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Revision as of 08:00, 13 December 2017

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NMR solution structure of hen lysozyme

Structural highlights

1e8l is a 1 chain structure with sequence from Chick. This structure supersedes the now removed PDB entry 1hwa. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	193l, 194l, 1a2y, 1aki, 1at5, 1at6, 1azf, 1b0d, 1bvx, 1bwh, 1bwi, 1bwj, 1c08, 1c10, 1dpw, 1dpx, 1dqj, 1f0w, 1f10, 1jpo, 1kip, 1kiq, 1kir, 1kxw, 1kxx, 1kxy, 1lcn, 1lkr, 1lks, 1lpi, 1lyo, 1lz8, 1lzn, 1mel, 1qtk, 1rfp, 1uco, 1uia, 1uib, 1uic, 1uid, 1uie, 1uif, 1uig, 1uih, 1xei, 1xej, 1xek, 2lyo, 3lyo, 3lzt, 4lyo, 4lzt
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Warning: this is a large structure, and loading might take a long time or not happen at all.

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

A high resolution NMR structure of hen lysozyme has been determined using 209 residual 1H-15N dipolar coupling restraints from measurements made in two different dilute liquid crystalline phases (bicelles) in conjunction with a data set of 1632 NOE distance restraints, 110 torsion angle restraints, and 60 hydrogen bond restraints. The ensemble of 50 low-energy calculated structures has an average backbone RMSD of 0.50+/-0.13A to the mean structure and of 1.49+/-0.10A to the crystal structure of hen lysozyme. To assess the importance of the dipolar coupling data in the structure determination, the final structures are compared with an ensemble calculated using an identical protocol but excluding the dipolar coupling restraints. The comparison shows that structures calculated with the dipolar coupling data are more similar to the crystal structure than those calculated without, and have better stereochemical quality. The structures also show improved quality factors when compared with additional dipolar coupling data that were not included in the structure calculations, with orientation-dependent 15N chemical shift changes measured in the bicelle solutions, and with T1/T2 values obtained from 15N relaxation measurements. Analysis of the ensemble of NMR structures and comparisons with crystal structures, 15N relaxation data, and molecular dynamics simulations of hen lysozyme provides a detailed description of the solution structure of this protein and insights into its dynamical behavior.

A refined solution structure of hen lysozyme determined using residual dipolar coupling data.,Schwalbe H, Grimshaw SB, Spencer A, Buck M, Boyd J, Dobson CM, Redfield C, Smith LJ Protein Sci. 2001 Apr;10(4):677-88. PMID:11274458^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Schwalbe H, Grimshaw SB, Spencer A, Buck M, Boyd J, Dobson CM, Redfield C, Smith LJ. A refined solution structure of hen lysozyme determined using residual dipolar coupling data. Protein Sci. 2001 Apr;10(4):677-88. PMID:11274458

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1e8l"

@@ Line 1: / Line 1: @@
-==NMR SOLUTION STRUCTURE OF HEN LYSOZYME==
+{{Large structure}}
+==NMR solution structure of hen lysozyme==
 <StructureSection load='1e8l' size='340' side='right' caption='[[1e8l]], [[NMR_Ensembles_of_Models | 50 NMR models]]' scene=''>
 == Structural highlights ==
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[193l|193l]], [[194l|194l]], [[1a2y|1a2y]], [[1aki|1aki]], [[1at5|1at5]], [[1at6|1at6]], [[1azf|1azf]], [[1b0d|1b0d]], [[1bvx|1bvx]], [[1bwh|1bwh]], [[1bwi|1bwi]], [[1bwj|1bwj]], [[1c08|1c08]], [[1c10|1c10]], [[1dpw|1dpw]], [[1dpx|1dpx]], [[1dqj|1dqj]], [[1f0w|1f0w]], [[1f10|1f10]], [[1jpo|1jpo]], [[1kip|1kip]], [[1kiq|1kiq]], [[1kir|1kir]], [[1kxw|1kxw]], [[1kxx|1kxx]], [[1kxy|1kxy]], [[1lcn|1lcn]], [[1lkr|1lkr]], [[1lks|1lks]], [[1lpi|1lpi]], [[1lyo|1lyo]], [[1lz8|1lz8]], [[1lzn|1lzn]], [[1mel|1mel]], [[1qtk|1qtk]], [[1rfp|1rfp]], [[1uco|1uco]], [[1uia|1uia]], [[1uib|1uib]], [[1uic|1uic]], [[1uid|1uid]], [[1uie|1uie]], [[1uif|1uif]], [[1uig|1uig]], [[1uih|1uih]], [[1xei|1xei]], [[1xej|1xej]], [[1xek|1xek]], [[2lyo|2lyo]], [[3lyo|3lyo]], [[3lzt|3lzt]], [[4lyo|4lyo]], [[4lzt|4lzt]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e8l OCA], [http://pdbe.org/1e8l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e8l RCSB], [http://www.ebi.ac.uk/pdbsum/1e8l PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e8l OCA], [http://pdbe.org/1e8l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e8l RCSB], [http://www.ebi.ac.uk/pdbsum/1e8l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e8l ProSAT]</span></td></tr>
 </table>
 {{Large structure}}
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e8/1e8l_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e8/1e8l_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1e8l

From Proteopedia

Revision as of 08:00, 13 December 2017

NMR solution structure of hen lysozyme

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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Proteopedia Page Contributors and Editors (what is this?)

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