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5odf
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==NtMe polyamide in complex with 5'CGATGTACATCG3'- hairpin polyamides studies== | |
| + | <StructureSection load='5odf' size='340' side='right' caption='[[5odf]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5odf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ODF FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CWN:NtMe+polyamide'>CWN</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DCZ:2-DEOXYCYTIDINE'>DCZ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5odf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odf OCA], [http://pdbe.org/5odf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5odf RCSB], [http://www.ebi.ac.uk/pdbsum/5odf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5odf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | This manuscript reports the molecular basis for double-stranded DNA (dsDNA) binding of hairpin polyamides incorporating a 5-alkyl thiazole (Nt) unit. Hairpin polyamides containing an N-terminal Nt unit induce higher melting stabilisation of target dsDNA sequences relative to an archetypical hairpin polyamide incorporating an N-terminal imidazole (Im) unit. However, modification of the N-terminus from Im to Nt-building blocks results in an increase in dsDNA binding affinity but lower G-selectivity. A general G-selectivity trend is observed for Nt-containing polyamide analogues. G-selectivity increases as the steric bulk in the Nt 5-position increases. Solution-based NMR structural studies reveal differences in the modulation of the target DNA duplex of Nt-containing hairpin polyamides relative to the Im-containing archetype. A structural hallmark of an Nt polyamide*dsDNA complex is a more significant degree of major groove compression of the target dsDNA sequence relative to the Im-containing hairpin polyamide. | ||
| - | + | Structural basis of DNA duplex distortion induced by thiazole-containing hairpin polyamides.,Padroni G, Parkinson JA, Fox KR, Burley GA Nucleic Acids Res. 2017 Nov 29. pii: 4670956. doi: 10.1093/nar/gkx1211. PMID:29194552<ref>PMID:29194552</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5odf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Burley, G A]] | ||
| + | [[Category: Padroni, G]] | ||
| + | [[Category: Parkinson, J]] | ||
| + | [[Category: Dna]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Hairpin polyamide]] | ||
| + | [[Category: Minor groove binder]] | ||
Revision as of 06:12, 20 December 2017
NtMe polyamide in complex with 5'CGATGTACATCG3'- hairpin polyamides studies
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