This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2cjx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 4: Line 4:
|PDB= 2cjx |SIZE=350|CAPTION= <scene name='initialview01'>2cjx</scene>, resolution 1.70&Aring;
|PDB= 2cjx |SIZE=350|CAPTION= <scene name='initialview01'>2cjx</scene>, resolution 1.70&Aring;
|SITE=
|SITE=
-
|LIGAND=
+
|LIGAND= <scene name='pdbligand=ASK:DEHYDROXYMETHYLASPARTIC+ACID'>ASK</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
 +
|DOMAIN=
 +
|RELATEDENTRY=
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjx OCA], [http://www.ebi.ac.uk/pdbsum/2cjx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cjx RCSB]</span>
}}
}}
Line 43: Line 46:
[[Category: zymogen]]
[[Category: zymogen]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:16:20 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:22:40 2008''

Revision as of 23:22, 30 March 2008

Image:2cjx.gif


PDB ID 2cjx

Drag the structure with the mouse to rotate
, resolution 1.70Å
Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



EXTENDED SUBSTRATE RECOGNITION IN CASPASE-3 REVEALED BY HIGH RESOLUTION X-RAY STRUCTURE ANALYSIS


Overview

Caspases are cysteine proteases involved in the signalling cascades of programmed cell death in which caspase-3 plays a central role, since it propagates death signals from intrinsic and extrinsic stimuli to downstream targets. The atomic resolution (1.06 Angstroms) crystal structure of the caspase-3 DEVD-cmk complex reveals the structural basis for substrate selectivity in the S4 pocket. A low-barrier hydrogen bond is observed between the side-chains of the P4 inhibitor aspartic acid and Asp179 of the N-terminal tail of the symmetry related p12 subunit. Site-directed mutagenesis of Asp179 confirmed the significance of this residue in substrate recognition. In the 1.06 Angstroms crystal structure, a radiation damage induced rearrangement of the inhibitor methylketone moiety was observed. The carbon atom that in a substrate would represent the scissile peptide bond carbonyl carbon clearly shows a tetrahedral coordination and resembles the postulated tetrahedral intermediate of the acylation reaction.

About this Structure

2CJX is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis., Ganesan R, Mittl PR, Jelakovic S, Grutter MG, J Mol Biol. 2006 Jun 23;359(5):1378-88. Epub 2006 May 11. PMID:16787777

Page seeded by OCA on Mon Mar 31 02:22:40 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cjx"
Personal tools