2cl6
From Proteopedia
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|PDB= 2cl6 |SIZE=350|CAPTION= <scene name='initialview01'>2cl6</scene>, resolution 1.24Å | |PDB= 2cl6 |SIZE=350|CAPTION= <scene name='initialview01'>2cl6</scene>, resolution 1.24Å | ||
|SITE= <scene name='pdbsite=AC1:Xy2+Binding+Site+For+Chain+X'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Xy2+Binding+Site+For+Chain+X'>AC1</scene> | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=CAG:GUANOSINE+5'-TRIPHOSPHATE+P3-[1-(2-NITROPHENYL)ETHYL+ESTER]'>CAG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=XY2:N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE'>XY2</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cl6 OCA], [http://www.ebi.ac.uk/pdbsum/2cl6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cl6 RCSB]</span> | ||
}} | }} | ||
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==Overview== | ==Overview== | ||
We present a new design for a fluorescence microspectrophotometer for use in kinetic crystallography in combination with x-ray diffraction experiments. The FLUMIX device (Fluorescence spectroscopy to monitor intermediates in x-ray crystallography) is built for 0 degrees fluorescence detection, which has several advantages in comparison to a conventional fluorometer with 90 degrees design. Due to the reduced spatial requirements and the need for only one objective, the system is highly versatile, easy to handle, and can be used for many different applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly in a hanging drop crystallization setup. The FLUMIX device can be combined with most x-ray sources, normally without the need of a specialized mechanical support. As a biological model system, we have used H-Ras p21 with an artificially introduced photo-labile GTP precursor (caged GTP) and a covalently attached fluorophore (IANBD amide). Using the FLUMIX system, detailed information about the state of photolyzed crystals of the modified H-Ras p21 (p21(mod)) could be obtained. Measurements in combination with a synchrotron beamline showed significant fluorescence changes in p21(mod) crystals even within a few seconds of x-ray exposure at 100 K. | We present a new design for a fluorescence microspectrophotometer for use in kinetic crystallography in combination with x-ray diffraction experiments. The FLUMIX device (Fluorescence spectroscopy to monitor intermediates in x-ray crystallography) is built for 0 degrees fluorescence detection, which has several advantages in comparison to a conventional fluorometer with 90 degrees design. Due to the reduced spatial requirements and the need for only one objective, the system is highly versatile, easy to handle, and can be used for many different applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly in a hanging drop crystallization setup. The FLUMIX device can be combined with most x-ray sources, normally without the need of a specialized mechanical support. As a biological model system, we have used H-Ras p21 with an artificially introduced photo-labile GTP precursor (caged GTP) and a covalently attached fluorophore (IANBD amide). Using the FLUMIX system, detailed information about the state of photolyzed crystals of the modified H-Ras p21 (p21(mod)) could be obtained. Measurements in combination with a synchrotron beamline showed significant fluorescence changes in p21(mod) crystals even within a few seconds of x-ray exposure at 100 K. | ||
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| - | ==Disease== | ||
| - | Known diseases associated with this structure: Bladder cancer, somatic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=190020 190020]], Costello syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=190020 190020]], Thyroid carcinoma, follicular, somatic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=190020 190020]] | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Klink, B U.]] | [[Category: Klink, B U.]] | ||
[[Category: Scheidig, A J.]] | [[Category: Scheidig, A J.]] | ||
| - | [[Category: CAG]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: XY2]] | ||
[[Category: disease mutation]] | [[Category: disease mutation]] | ||
[[Category: fluorescence]] | [[Category: fluorescence]] | ||
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[[Category: proto-oncogene]] | [[Category: proto-oncogene]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:23:01 2008'' |
Revision as of 23:23, 30 March 2008
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| , resolution 1.24Å | |||||||
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| Sites: | |||||||
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH S-CAGED GTP
Overview
We present a new design for a fluorescence microspectrophotometer for use in kinetic crystallography in combination with x-ray diffraction experiments. The FLUMIX device (Fluorescence spectroscopy to monitor intermediates in x-ray crystallography) is built for 0 degrees fluorescence detection, which has several advantages in comparison to a conventional fluorometer with 90 degrees design. Due to the reduced spatial requirements and the need for only one objective, the system is highly versatile, easy to handle, and can be used for many different applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly in a hanging drop crystallization setup. The FLUMIX device can be combined with most x-ray sources, normally without the need of a specialized mechanical support. As a biological model system, we have used H-Ras p21 with an artificially introduced photo-labile GTP precursor (caged GTP) and a covalently attached fluorophore (IANBD amide). Using the FLUMIX system, detailed information about the state of photolyzed crystals of the modified H-Ras p21 (p21(mod)) could be obtained. Measurements in combination with a synchrotron beamline showed significant fluorescence changes in p21(mod) crystals even within a few seconds of x-ray exposure at 100 K.
About this Structure
2CL6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction., Klink BU, Goody RS, Scheidig AJ, Biophys J. 2006 Aug 1;91(3):981-92. Epub 2006 May 12. PMID:16698776
Page seeded by OCA on Mon Mar 31 02:23:01 2008
Categories: Homo sapiens | Single protein | Goody, R S. | Klink, B U. | Scheidig, A J. | Disease mutation | Fluorescence | Golgi apparatus | Gppnhp | Gtp-binding | Guanine nucleotide binding protein | Lipoprotein | Membrane | Methylation | Nucleotide-binding | Palmitate | Prenylation | Proto-oncogene
