6en7
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the ribosome assembly factor Nsa1== | |
| + | <StructureSection load='6en7' size='340' side='right' caption='[[6en7]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6en7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EN7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EN7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6en7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6en7 OCA], [http://pdbe.org/6en7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6en7 RCSB], [http://www.ebi.ac.uk/pdbsum/6en7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6en7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/NSA1_YEAST NSA1_YEAST]] Involved in the biogenesis of the 60S ribosomal subunit.<ref>PMID:16544271</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and approximately 50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural information, this process is poorly understood. Using cryo-EM, we solved structures of early 60S biogenesis intermediates at 3.3 A to 4.5 A resolution, thereby providing insights into their sequential folding and assembly pathway. Besides revealing distinct immature rRNA conformations, we map 25 assembly factors in six different assembly states. Notably, the Nsa1-Rrp1-Rpf1-Mak16 module stabilizes the solvent side of the 60S subunit, and the Erb1-Ytm1-Nop7 complex organizes and connects through Erb1's meandering N-terminal extension, eight assembly factors, three ribosomal proteins, and three 25S rRNA domains. Our structural snapshots reveal the order of integration and compaction of the six major 60S domains within early nucleolar 60S particles developing stepwise from the solvent side around the exit tunnel to the central protuberance. | ||
| - | + | Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.,Kater L, Thoms M, Barrio-Garcia C, Cheng J, Ismail S, Ahmed YL, Bange G, Kressler D, Berninghausen O, Sinning I, Hurt E, Beckmann R Cell. 2017 Dec 14;171(7):1599-1610.e14. doi: 10.1016/j.cell.2017.11.039. PMID:29245012<ref>PMID:29245012</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 6en7" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Altegoer, F]] | [[Category: Altegoer, F]] | ||
| + | [[Category: Bange, G]] | ||
| + | [[Category: Biogenesis]] | ||
| + | [[Category: Chaperone]] | ||
| + | [[Category: Ribosome]] | ||
Revision as of 08:42, 27 December 2017
Crystal structure of the ribosome assembly factor Nsa1
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Categories: Altegoer, F | Bange, G | Biogenesis | Chaperone | Ribosome
