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5oqn

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Revision as of 08:54, 27 December 2017

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (short kleisin loop)

Structural highlights

5oqn is a 4 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	YCG1, YCS5, YDR325W (Baker's yeast), BRN1, YBL097W, YBL0830 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CND3_YEAST] Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase. [CND2_YEAST] Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase.^[1] ^[2]

Publication Abstract from PubMed

Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin rearrangements that take place during cell divisions. Here, we identify a direct DNA-binding site in the eukaryotic condensin complex, which is formed by its Ycg1Cnd3 HEAT-repeat and Brn1Cnd2 kleisin subunits. DNA co-crystal structures reveal a conserved, positively charged groove that accommodates the DNA double helix. A peptide loop of the kleisin subunit encircles the bound DNA and, like a safety belt, prevents its dissociation. Firm closure of the kleisin loop around DNA is essential for the association of condensin complexes with chromosomes and their DNA-stimulated ATPase activity. Our data suggest a sophisticated molecular basis for anchoring condensin complexes to chromosomes that enables the formation of large-sized chromatin loops.

Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.,Kschonsak M, Merkel F, Bisht S, Metz J, Rybin V, Hassler M, Haering CH Cell. 2017 Oct 4. pii: S0092-8674(17)31057-7. doi: 10.1016/j.cell.2017.09.008. PMID:28988770^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lavoie BD, Tuffo KM, Oh S, Koshland D, Holm C. Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren. Mol Biol Cell. 2000 Apr;11(4):1293-304. PMID:10749930
↑ Ouspenski II, Cabello OA, Brinkley BR. Chromosome condensation factor Brn1p is required for chromatid separation in mitosis. Mol Biol Cell. 2000 Apr;11(4):1305-13. PMID:10749931
↑ Kschonsak M, Merkel F, Bisht S, Metz J, Rybin V, Hassler M, Haering CH. Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes. Cell. 2017 Oct 4. pii: S0092-8674(17)31057-7. doi: 10.1016/j.cell.2017.09.008. PMID:28988770 doi:http://dx.doi.org/10.1016/j.cell.2017.09.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5oqn"

@@ Line 3: / Line 3: @@
 <StructureSection load='5oqn' size='340' side='right' caption='[[5oqn]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5oqn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5oqn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQN FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqn OCA], [http://pdbe.org/5oqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oqn RCSB], [http://www.ebi.ac.uk/pdbsum/5oqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqn ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YCG1, YCS5, YDR325W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), BRN1, YBL097W, YBL0830 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqn OCA], [http://pdbe.org/5oqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oqn RCSB], [http://www.ebi.ac.uk/pdbsum/5oqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqn ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
 [[Category: Haering, C H]]
 [[Category: Hassler, M]]

5oqn

From Proteopedia

Revision as of 08:54, 27 December 2017

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (short kleisin loop)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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