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1fkm

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Revision as of 09:23, 27 December 2017

CRYSTAL STRUCTURE OF THE YPT/RAB-GAP DOMAIN OF GYP1P

Structural highlights

1fkm is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	1ek0
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GYP1_YEAST] Stimulates specifically the GTPase activity of YPT1. Functions on the Golgi as a negative regulator of YPT1.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We present the 1.9 A resolution crystal structure of the catalytic domain of Gyp1p, a specific GTPase activating protein (GAP) for Ypt proteins, the yeast homologues of Rab proteins, which are involved in vesicular transport. Gyp1p is a member of a large family of eukaryotic proteins with shared sequence motifs. Previously, no structural information was available for any member of this class of proteins. The GAP domain of Gyp1p was found to be fully alpha-helical. However, the observed fold does not superimpose with other alpha-helical GAPs (e.g. Ras- and Cdc42/Rho-GAP). The conserved and catalytically crucial arginine residue, identified by mutational analysis, is in a comparable position to the arginine finger in the Ras- and Cdc42-GAPs, suggesting that Gyp1p utilizes an arginine finger in the GAP reaction, in analogy to Ras- and Cdc42-GAPs. A model for the interaction between Gyp1p and the Ypt protein satisfying biochemical data is given.

Crystal structure of the GAP domain of Gyp1p: first insights into interaction with Ypt/Rab proteins.,Rak A, Fedorov R, Alexandrov K, Albert S, Goody RS, Gallwitz D, Scheidig AJ EMBO J. 2000 Oct 2;19(19):5105-13. PMID:11013213^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Du LL, Novick P. Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1p. Mol Biol Cell. 2001 May;12(5):1215-26. PMID:11359917
↑ Rak A, Fedorov R, Alexandrov K, Albert S, Goody RS, Gallwitz D, Scheidig AJ. Crystal structure of the GAP domain of Gyp1p: first insights into interaction with Ypt/Rab proteins. EMBO J. 2000 Oct 2;19(19):5105-13. PMID:11013213 doi:10.1093/emboj/19.19.5105

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fkm"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF THE YPT/RAB-GAP DOMAIN OF GYP1P==
 <StructureSection load='1fkm' size='340' side='right' caption='[[1fkm]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ek0|1ek0]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkm OCA], [http://pdbe.org/1fkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fkm RCSB], [http://www.ebi.ac.uk/pdbsum/1fkm PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkm OCA], [http://pdbe.org/1fkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fkm RCSB], [http://www.ebi.ac.uk/pdbsum/1fkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkm ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/1fkm_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/1fkm_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1fkm

From Proteopedia

Revision as of 09:23, 27 December 2017

CRYSTAL STRUCTURE OF THE YPT/RAB-GAP DOMAIN OF GYP1P

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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