5w2d
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of mutant CJ YCEI protein (CJ-G34C) for nanotechnology applications== | |
| + | <StructureSection load='5w2d' size='340' side='right' caption='[[5w2d]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5w2d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W2D FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w17|5w17]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w2d OCA], [http://pdbe.org/5w2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w2d RCSB], [http://www.ebi.ac.uk/pdbsum/5w2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w2d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (>13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals. | ||
| - | + | Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.,Huber TR, McPherson EC, Keating CE, Snow CD Bioconjug Chem. 2017 Dec 20. doi: 10.1021/acs.bioconjchem.7b00668. PMID:29232505<ref>PMID:29232505</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Huber, T | + | <div class="pdbe-citations 5w2d" style="background-color:#fffaf0;"></div> |
| - | [[Category: Snow, C | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Huber, T R]] | ||
| + | [[Category: Snow, C D]] | ||
| + | [[Category: Nanomaterial nanoporous]] | ||
| + | [[Category: Unknown function]] | ||
Revision as of 05:46, 3 January 2018
Crystal structure of mutant CJ YCEI protein (CJ-G34C) for nanotechnology applications
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