2czr
From Proteopedia
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|PDB= 2czr |SIZE=350|CAPTION= <scene name='initialview01'>2czr</scene>, resolution 2.30Å | |PDB= 2czr |SIZE=350|CAPTION= <scene name='initialview01'>2czr</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2czr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czr OCA], [http://www.ebi.ac.uk/pdbsum/2czr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2czr RCSB]</span> | ||
}} | }} | ||
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[[Category: Morikawa, M.]] | [[Category: Morikawa, M.]] | ||
[[Category: Yamamoto, T.]] | [[Category: Yamamoto, T.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: ZN]] | ||
[[Category: hyperthermophilic archaeon]] | [[Category: hyperthermophilic archaeon]] | ||
[[Category: tata-binding protein (tbp)]] | [[Category: tata-binding protein (tbp)]] | ||
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[[Category: zn-finger motif]] | [[Category: zn-finger motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:28:24 2008'' |
Revision as of 23:28, 30 March 2008
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| , resolution 2.30Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA
Overview
TATA-binding protein (TBP)-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1 (Tk-TIP26) is a possible transcription regulatory protein in Thermococcales. Here, we report the crystal structure of Tk-TIP26 determined at 2.3 A resolution with multiple-wavelength anomalous dispersion (MAD) method. The overall structure of Tk-TIP26 consists of two domains. The N-terminal domain forms an alpha/beta structure, in which three alpha-helices enclose the central beta-sheet. The topology of this domain is similar to that of holliday junction resolvase Hjc from Pyrococcus furiosus. The C-terminal domain comprises three alpha-helices, six beta-strands, and two 3(10)-helices. In the dimer structure of Tk-TIP26, two molecules are related with the crystallographic twofold axis, and these molecules rigidly interact with each other via hydrogen bonds. The complex of Tk-TIP26/Tk-TBP is isolated and analyzed by SDS-PAGE and gel filtration column chromatography, resulting in a stoichiometric ratio of the interaction between Tk-TIP26 and Tk-TBP of 4:2, i.e., two dimer molecules of Tk-TIP26 formed a complex with one dimeric TBP. The electrostatic surfaces of Tk-TIP26 and TBP from Pyrocuccus woesei (PwTBP) allowed us to build a model of the Tk-TIP26/TBP complex, and to propose the inhibition mechanism where two dimer molecules of Tk-TIP26 bind to a dimeric TBP, preventing its binding to TATA-DNA.
About this Structure
2CZR is a Single protein structure of sequence from Thermococcus kodakarensis. Full crystallographic information is available from OCA.
Reference
Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA., Yamamoto T, Matsuda T, Inoue T, Matsumura H, Morikawa M, Kanaya S, Kai Y, Protein Sci. 2006 Jan;15(1):152-61. Epub 2005 Dec 1. PMID:16322571
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