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5ujy
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of Mycobacterium tuberculosis topoisomerase I from the 2nd crystal form== |
| - | + | <StructureSection load='5ujy' size='340' side='right' caption='[[5ujy]], [[Resolution|resolution]] 2.70Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[5ujy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UJY FirstGlance]. <br> | |
| - | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d5h|5d5h]], [[5uj1|5uj1]], [[5ukt|5ukt]]</td></tr> | |
| - | [[Category: | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr> |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ujy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ujy OCA], [http://pdbe.org/5ujy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ujy RCSB], [http://www.ebi.ac.uk/pdbsum/5ujy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ujy ProSAT]</span></td></tr> |
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/A0A0E8VY41_MYCTX A0A0E8VY41_MYCTX]] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952] | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: DNA topoisomerase]] | ||
| + | [[Category: Cao, N]] | ||
[[Category: Tan, K]] | [[Category: Tan, K]] | ||
| - | [[Category: | + | [[Category: Tse-Dinh, Y C]] |
| + | [[Category: Dna topoisomerase i]] | ||
| + | [[Category: Isomerase]] | ||
Revision as of 06:52, 17 January 2018
The structure of Mycobacterium tuberculosis topoisomerase I from the 2nd crystal form
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