5y00
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Acid-tolerant monomeric GFP, Gamillus, fluorescence (ON) state== | |
| + | <StructureSection load='5y00' size='340' side='right' caption='[[5y00]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5y00]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y00 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y00 OCA], [http://pdbe.org/5y00 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y00 RCSB], [http://www.ebi.ac.uk/pdbsum/5y00 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y00 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The fluorescent protein (FP) color palette has greatly contributed to the visualization of molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than their neutral pKa ( approximately 6), and this has hampered their application in acidic organelles (pH approximately 4.5-6.0). Currently, several cyan- and red-colored acid-tolerant FPs are available; however, there are few reports of acid-tolerant green FPs (GFPs) that are practically applicable to bioimaging. Here, we developed the acid-tolerant monomeric GFP "Gamillus" from the jellyfish Olindias formosa, with excellent brightness, maturation speed, and photostability. Results from X-ray crystallography and point mutagenesis suggest that across a broad pH range the acid tolerance is attributed to stabilization of deprotonation in the chromophore phenyl ring by forming a unique trans configuration. We demonstrate that Gamillus can serve as a molecular tag suitable for imaging in acidic organelles through autophagy-mediated molecular tracking to lysosomes. | ||
| - | + | Acid-Tolerant Monomeric GFP from Olindias formosa.,Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T Cell Chem Biol. 2017 Dec 23. pii: S2451-9456(17)30432-4. doi:, 10.1016/j.chembiol.2017.12.005. PMID:29290624<ref>PMID:29290624</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5y00" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Matsuda, T]] | [[Category: Matsuda, T]] | ||
| - | [[Category: Sakurai, K]] | ||
| - | [[Category: Nakashima, R]] | ||
[[Category: Nagai, T]] | [[Category: Nagai, T]] | ||
| + | [[Category: Nakashima, R]] | ||
| + | [[Category: Sakurai, K]] | ||
| + | [[Category: Shinoda, H]] | ||
| + | [[Category: Beta barrel]] | ||
| + | [[Category: Fluorescent protein]] | ||
| + | [[Category: Gfp-like protein]] | ||
| + | [[Category: Trans-chromophore]] | ||
Revision as of 06:57, 17 January 2018
Acid-tolerant monomeric GFP, Gamillus, fluorescence (ON) state
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