6c0w
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of human kinetochore protein CENP-N with the centromeric nucleosome containing CENP-A== | |
| + | <StructureSection load='6c0w' size='340' side='right' caption='[[6c0w]], [[Resolution|resolution]] 4.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6c0w]] is a 11 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C0W FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6eqt|6eqt]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c0w OCA], [http://pdbe.org/6c0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c0w RCSB], [http://www.ebi.ac.uk/pdbsum/6c0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c0w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/CENPA_HUMAN CENPA_HUMAN]] Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro).<ref>PMID:20739937</ref> <ref>PMID:21478274</ref> [[http://www.uniprot.org/uniprot/H2A1C_HUMAN H2A1C_HUMAN]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [[http://www.uniprot.org/uniprot/CENPN_HUMAN CENPN_HUMAN]] Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.<ref>PMID:16622419</ref> <ref>PMID:16716197</ref> <ref>PMID:18007590</ref> <ref>PMID:19543270</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Centromere protein (CENP) A, a histone H3 variant, is a key epigenetic determinant of chromosome domains known as centromeres. Centromeres nucleate kinetochores, multi-subunit complexes that capture spindle microtubules to promote chromosome segregation during mitosis. Two kinetochore proteins, CENP-C and CENP-N, recognize CENP-A in the context of a rare CENP-A nucleosome. Here, we reveal the structural basis for the exquisite selectivity of CENP-N for centromeres. CENP-N uses charge and space complementarity to decode the L1 loop that is unique to CENP-A. It also engages in extensive interactions with a 15-base pair segment of the distorted nucleosomal DNA double helix, in a position predicted to exclude chromatin remodelling enzymes. Besides CENP-A, stable centromere recruitment of CENP-N requires a coincident interaction with a newly identified binding motif on nucleosome-bound CENP-C. Collectively, our studies clarify how CENP-N and CENP-C decode and stabilize the non-canonical CENP-A nucleosome to enforce epigenetic centromere specification and kinetochore assembly. | ||
| - | + | Decoding the centromeric nucleosome through CENP-N.,Pentakota S, Zhou K, Smith C, Maffini S, Petrovic A, Morgan GP, Weir JR, Vetter IR, Musacchio A, Luger K Elife. 2017 Dec 27;6. doi: 10.7554/eLife.33442. PMID:29280735<ref>PMID:29280735</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 6c0w" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Luger, K]] | [[Category: Luger, K]] | ||
| - | + | [[Category: Morgan, G P]] | |
| - | + | ||
| - | [[Category: Morgan, G | + | |
[[Category: Musacchio, A]] | [[Category: Musacchio, A]] | ||
[[Category: Pentakota, S]] | [[Category: Pentakota, S]] | ||
| + | [[Category: Petrovic, A]] | ||
| + | [[Category: Vetter, I R]] | ||
| + | [[Category: Zhou, K]] | ||
| + | [[Category: Cenp-a]] | ||
| + | [[Category: Cenp-n]] | ||
| + | [[Category: Kinetochore]] | ||
| + | [[Category: Nucleosome]] | ||
| + | [[Category: Structural protein-dna complex]] | ||
Revision as of 07:03, 17 January 2018
Cryo-EM structure of human kinetochore protein CENP-N with the centromeric nucleosome containing CENP-A
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