2dj5
From Proteopedia
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|PDB= 2dj5 |SIZE=350|CAPTION= <scene name='initialview01'>2dj5</scene>, resolution 2.55Å | |PDB= 2dj5 |SIZE=350|CAPTION= <scene name='initialview01'>2dj5</scene>, resolution 2.55Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Sirohydrochlorin_cobaltochelatase Sirohydrochlorin cobaltochelatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.99.1.3 4.99.1.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Sirohydrochlorin_cobaltochelatase Sirohydrochlorin cobaltochelatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.99.1.3 4.99.1.3] </span> |
|GENE= cbiX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus]) | |GENE= cbiX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1tjn|1TJN]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dj5 OCA], [http://www.ebi.ac.uk/pdbsum/2dj5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dj5 RCSB]</span> | ||
}} | }} | ||
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[[Category: James, M N.G.]] | [[Category: James, M N.G.]] | ||
[[Category: Yin, J.]] | [[Category: Yin, J.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: PO4]] | ||
[[Category: beta-alpha-beta]] | [[Category: beta-alpha-beta]] | ||
[[Category: parallel beta sheet]] | [[Category: parallel beta sheet]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:35:18 2008'' |
Revision as of 23:35, 30 March 2008
| |||||||
| , resolution 2.55Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | cbiX (Archaeoglobus fulgidus) | ||||||
| Activity: | Sirohydrochlorin cobaltochelatase, with EC number 4.99.1.3 | ||||||
| Related: | 1TJN
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the vitamin B12 biosynthetic cobaltochelatase, CbiXS, from Archaeoglobus fulgidus
Overview
The Archaeoglobus fulgidus gene af0721 encodes CbiX(S), a small cobaltochelatase associated with the anaerobic biosynthesis of vitamin B12 (cobalamin). The protein was shown to have activity both in vivo and in vitro, catalyzing the insertion of Co2+ into sirohydrochlorin. The structure of CbiX(S) was determined in two different crystal forms and was shown to consist of a central mixed beta-sheet flanked by four alpha-helices, one of which originates in the C-terminus of a neighboring molecule. CbiX(S) is about half the size of other Class II tetrapyrrole chelatases. The overall topography of CbiX(S) exhibits substantial resemblance to both the N- and C-terminal regions of several members of the Class II metal chelatases involved in tetrapyrrole biosynthesis. Two histidines (His10 and His74), are in similar positions as the catalytic histidine residues in the anaerobic cobaltochelatase CbiK (His145 and His207). In light of the hypothesis that suggests the larger chelatases evolved via gene duplication and fusion from a CbiX(S)-like enzyme, the structure of AF0721 may represent that of an "ancestral" precursor of class II metal chelatases.
About this Structure
2DJ5 is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the vitamin B12 biosynthetic cobaltochelatase, CbiXS, from Archaeoglobus fulgidus., Yin J, Xu LX, Cherney MM, Raux-Deery E, Bindley AA, Savchenko A, Walker JR, Cuff ME, Warren MJ, James MN, J Struct Funct Genomics. 2006 Mar;7(1):37-50. Epub 2006 Jul 12. PMID:16835730
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