T7 RNA Polymerase
From Proteopedia
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==T7 RNA Polymerase Initiation Complex== | ==T7 RNA Polymerase Initiation Complex== | ||
<StructureSection load='1qln' size='450' side='right' caption='Promoter bound polymerase' scene=''> | <StructureSection load='1qln' size='450' side='right' caption='Promoter bound polymerase' scene=''> | ||
| - | This is a default text for your page '''T7 rna polymerase'''. Click above on '''edit this page''' to modify. Be careful with the < and > signs. | ||
| - | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
T7 RNA polymerase <scene name='77/778917/Basic_cartoon_view/1'>binds and melts into dsDNA</scene>, by recognizing the upstream duplex region of the promoter (-17 to -5), and then melting a bubble (-4 to about +3), within the larger duplex. The duplex promoter domain binds primarily to the N-terminal domain of the enzyme, with the exception of the (C-terminal domain) "specificity loop." It is the combination of the N-terminal domain, with the positioned specificity loop, that forms the specific binding surface. | T7 RNA polymerase <scene name='77/778917/Basic_cartoon_view/1'>binds and melts into dsDNA</scene>, by recognizing the upstream duplex region of the promoter (-17 to -5), and then melting a bubble (-4 to about +3), within the larger duplex. The duplex promoter domain binds primarily to the N-terminal domain of the enzyme, with the exception of the (C-terminal domain) "specificity loop." It is the combination of the N-terminal domain, with the positioned specificity loop, that forms the specific binding surface. | ||
The enzyme then binds the first two substrate NTP's, as directed by the template (typically two GTP's, encoded by CC in the template strand). A phosphoryl transfer reaction occurs to form the product dinucleotide (pppGpG). | The enzyme then binds the first two substrate NTP's, as directed by the template (typically two GTP's, encoded by CC in the template strand). A phosphoryl transfer reaction occurs to form the product dinucleotide (pppGpG). | ||
Revision as of 20:13, 21 January 2018
T7 RNA Polymerase Initiation Complex
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References
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