| Structural highlights
Function
[POLS_SINDV] Capsid protein possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. New virions attach to target cells, and after clathrin-mediated endocytosis their membrane fuses with the host endosomal membrane. This leads to the release of the nucleocapsid into the cytoplasm, followed by an uncoating event necessary for the genomic RNA to become accessible. The uncoating might be triggered by the interaction of capsid proteins with ribosomes. Binding of ribosomes would release the genomic RNA since the same region is genomic RNA-binding and ribosome-binding (By similarity).[1] [2] [3] [4] E3 protein's function is unknown (By similarity).[5] [6] [7] [8] E2 is responsible for viral attachment to target host cell, by binding to the cell receptor. Synthesized as a p62 precursor which is processed by furin at the cell membrane just before virion budding, giving rise to E2-E1 heterodimer. The p62-E1 heterodimer is stable, whereas E2-E1 is unstable and dissociate at low pH. p62 is processed at the last step, presumably to avoid E1 fusion activation before its final export to cell surface. E2 C-terminus contains a transitory transmembrane that would be disrupted by palmitoylation, resulting in reorientation of the C-terminal tail from lumenal to cytoplasmic side. This step is critical since E2 C-terminus is involved in budding by interacting with capsid proteins. This release of E2 C-terminus in cytoplasm occurs lately in protein export, and precludes premature assembly of particles at the endoplasmic reticulum membrane (By similarity).[9] [10] [11] [12] 6K is a constitutive membrane protein involved in virus glycoprotein processing, cell permeabilization, and the budding of viral particles. Disrupts the calcium homeostasis of the cell, probably at the endoplasmic reticulum level. This leads to cytoplasmic calcium elevation. Because of its lipophilic properties, the 6K protein is postulated to influence the selection of lipids that interact with the transmembrane domains of the glycoproteins, which, in turn, affects the deformability of the bilayer required for the extreme curvature that occurs as budding proceeds. Present in low amount in virions, about 3% compared to viral glycoproteins.[13] [14] [15] [16] E1 is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 trimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion. Efficient fusion requires the presence of cholesterol and sphingolipid in the target membrane (By similarity).[17] [18] [19] [20] [GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The structure of the lipid-enveloped Sindbis virus has been determined by fitting atomic resolution crystallographic structures of component proteins into an 11-A resolution cryoelectron microscopy map. The virus has T=4 quasisymmetry elements that are accurately maintained between the external glycoproteins, the transmembrane helical region, and the internal nucleocapsid core. The crystal structure of the E1 glycoprotein was fitted into the cryoelectron microscopy density, in part by using the known carbohydrate positions as restraints. A difference map showed that the E2 glycoprotein was shaped similarly to E1, suggesting a possible common evolutionary origin for these two glycoproteins. The structure shows that the E2 glycoprotein would have to move away from the center of the trimeric spike in order to expose enough viral membrane surface to permit fusion with the cellular membrane during the initial stages of host infection. The well-resolved E1-E2 transmembrane regions form alpha-helical coiled coils that were consistent with T=4 symmetry. The known structure of the capsid protein was fitted into the density corresponding to the nucleocapsid, revising the structure published earlier.
Placement of the structural proteins in Sindbis virus.,Zhang W, Mukhopadhyay S, Pletnev SV, Baker TS, Kuhn RJ, Rossmann MG J Virol. 2002 Nov;76(22):11645-58. PMID:12388725[21]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Smit JM, Bittman R, Wilschut J. Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J Virol. 1999 Oct;73(10):8476-84. PMID:10482600
- ↑ DeTulleo L, Kirchhausen T. The clathrin endocytic pathway in viral infection. EMBO J. 1998 Aug 17;17(16):4585-93. PMID:9707418 doi:10.1093/emboj/17.16.4585
- ↑ Sanz MA, Madan V, Carrasco L, Nieva JL. Interfacial domains in Sindbis virus 6K protein. Detection and functional characterization. J Biol Chem. 2003 Jan 17;278(3):2051-7. Epub 2002 Nov 6. PMID:12424249 doi:10.1074/jbc.M206611200
- ↑ Antoine AF, Montpellier C, Cailliau K, Browaeys-Poly E, Vilain JP, Dubuisson J. The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J Membr Biol. 2007 Jan;215(1):37-48. Epub 2007 May 5. PMID:17483865 doi:10.1007/s00232-007-9003-6
- ↑ Smit JM, Bittman R, Wilschut J. Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J Virol. 1999 Oct;73(10):8476-84. PMID:10482600
- ↑ DeTulleo L, Kirchhausen T. The clathrin endocytic pathway in viral infection. EMBO J. 1998 Aug 17;17(16):4585-93. PMID:9707418 doi:10.1093/emboj/17.16.4585
- ↑ Sanz MA, Madan V, Carrasco L, Nieva JL. Interfacial domains in Sindbis virus 6K protein. Detection and functional characterization. J Biol Chem. 2003 Jan 17;278(3):2051-7. Epub 2002 Nov 6. PMID:12424249 doi:10.1074/jbc.M206611200
- ↑ Antoine AF, Montpellier C, Cailliau K, Browaeys-Poly E, Vilain JP, Dubuisson J. The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J Membr Biol. 2007 Jan;215(1):37-48. Epub 2007 May 5. PMID:17483865 doi:10.1007/s00232-007-9003-6
- ↑ Smit JM, Bittman R, Wilschut J. Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J Virol. 1999 Oct;73(10):8476-84. PMID:10482600
- ↑ DeTulleo L, Kirchhausen T. The clathrin endocytic pathway in viral infection. EMBO J. 1998 Aug 17;17(16):4585-93. PMID:9707418 doi:10.1093/emboj/17.16.4585
- ↑ Sanz MA, Madan V, Carrasco L, Nieva JL. Interfacial domains in Sindbis virus 6K protein. Detection and functional characterization. J Biol Chem. 2003 Jan 17;278(3):2051-7. Epub 2002 Nov 6. PMID:12424249 doi:10.1074/jbc.M206611200
- ↑ Antoine AF, Montpellier C, Cailliau K, Browaeys-Poly E, Vilain JP, Dubuisson J. The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J Membr Biol. 2007 Jan;215(1):37-48. Epub 2007 May 5. PMID:17483865 doi:10.1007/s00232-007-9003-6
- ↑ Smit JM, Bittman R, Wilschut J. Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J Virol. 1999 Oct;73(10):8476-84. PMID:10482600
- ↑ DeTulleo L, Kirchhausen T. The clathrin endocytic pathway in viral infection. EMBO J. 1998 Aug 17;17(16):4585-93. PMID:9707418 doi:10.1093/emboj/17.16.4585
- ↑ Sanz MA, Madan V, Carrasco L, Nieva JL. Interfacial domains in Sindbis virus 6K protein. Detection and functional characterization. J Biol Chem. 2003 Jan 17;278(3):2051-7. Epub 2002 Nov 6. PMID:12424249 doi:10.1074/jbc.M206611200
- ↑ Antoine AF, Montpellier C, Cailliau K, Browaeys-Poly E, Vilain JP, Dubuisson J. The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J Membr Biol. 2007 Jan;215(1):37-48. Epub 2007 May 5. PMID:17483865 doi:10.1007/s00232-007-9003-6
- ↑ Smit JM, Bittman R, Wilschut J. Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J Virol. 1999 Oct;73(10):8476-84. PMID:10482600
- ↑ DeTulleo L, Kirchhausen T. The clathrin endocytic pathway in viral infection. EMBO J. 1998 Aug 17;17(16):4585-93. PMID:9707418 doi:10.1093/emboj/17.16.4585
- ↑ Sanz MA, Madan V, Carrasco L, Nieva JL. Interfacial domains in Sindbis virus 6K protein. Detection and functional characterization. J Biol Chem. 2003 Jan 17;278(3):2051-7. Epub 2002 Nov 6. PMID:12424249 doi:10.1074/jbc.M206611200
- ↑ Antoine AF, Montpellier C, Cailliau K, Browaeys-Poly E, Vilain JP, Dubuisson J. The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J Membr Biol. 2007 Jan;215(1):37-48. Epub 2007 May 5. PMID:17483865 doi:10.1007/s00232-007-9003-6
- ↑ Zhang W, Mukhopadhyay S, Pletnev SV, Baker TS, Kuhn RJ, Rossmann MG. Placement of the structural proteins in Sindbis virus. J Virol. 2002 Nov;76(22):11645-58. PMID:12388725
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