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1kk0

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Revision as of 21:37, 24 January 2018

Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi

Structural highlights

1kk0 is a 1 chain structure with sequence from "pyrococcus_abyssi"_erauso_et_al._1993 "pyrococcus abyssi" erauso et al. 1993. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Related:	1kjz
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IF2G_PYRAB] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal initiation of translation. By delivering the initiator methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation codon selection. The three subunits of aIF2 from the hyperthermophilic archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The beta and gamma subunits each contain a tightly bound zinc. The large gamma subunit is shown to form the structural core for trimer assembly. The crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma displays marked similarities to elongation factors. A distinctive feature of e/aIF2gamma is a subdomain containing a zinc-binding knuckle. Examination of the nucleotide-complexed aIF2gamma structures suggests mechanisms of action and tRNA binding properties similar to those of an elongation factor. Implications for the mechanism of translation initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the search for the initiation codon is envisaged.

The large subunit of initiation factor aIF2 is a close structural homologue of elongation factors.,Schmitt E, Blanquet S, Mechulam Y EMBO J. 2002 Apr 2;21(7):1821-32. PMID:11927566^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schmitt E, Blanquet S, Mechulam Y. The large subunit of initiation factor aIF2 is a close structural homologue of elongation factors. EMBO J. 2002 Apr 2;21(7):1821-32. PMID:11927566 doi:10.1093/emboj/21.7.1821

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kk0"

@@ Line 1: / Line 1: @@
 ==Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi==
 <StructureSection load='1kk0' size='340' side='right' caption='[[1kk0]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kjz|1kjz]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk0 OCA], [http://pdbe.org/1kk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kk0 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk0 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk0 OCA], [http://pdbe.org/1kk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kk0 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kk0 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/1kk0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/1kk0_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1kk0

From Proteopedia

Revision as of 21:37, 24 January 2018

Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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