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5wj4

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Revision as of 07:08, 31 January 2018

Crystal structure of redox-sensitive green fluorescent protein Clover mutant roClover1

Structural highlights

5wj4 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Publication Abstract from PubMed

We have determined the crystal structure of Clover, one of the brightest fluorescent proteins, and found that its T203H/S65G mutations relative to wild-type GFP lock the critical E222 side chain in a fixed configuration that mimics the major conformer of that in EGFP. The resulting equilibrium shift to the predominantly deprotonated chromophore increases the extinction coefficient (EC), opposes photoactivation, and is responsible for the bathochromic shift. Clover's brightness can further be attributed to a pi-pi stacking interaction between H203 and the chromophore. Consistent with these observations, the Clover G65S mutant reversed the equilibrium shift, dramatically decreased the EC, and made Clover photoactivatable under conditions that activated photoactivatable GFP. Using the Clover structure, we rationally engineered a non-photoactivatable redox sensor, roClover1, and determined its structure as well as that of its parental template, roClover0.1. These high-resolution structures provide deeper insights into structure-function relationships in GFPs and may aid the development of excitation-improved ratiometric biosensors.

Crystal Structure of Green Fluorescent Protein Clover and Design of Clover-Based Redox Sensors.,Campbell BC, Petsko GA, Liu CF Structure. 2017 Dec 27. pii: S0969-2126(17)30405-7. doi:, 10.1016/j.str.2017.12.006. PMID:29307487^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Campbell BC, Petsko GA, Liu CF. Crystal Structure of Green Fluorescent Protein Clover and Design of Clover-Based Redox Sensors. Structure. 2017 Dec 27. pii: S0969-2126(17)30405-7. doi:, 10.1016/j.str.2017.12.006. PMID:29307487 doi:http://dx.doi.org/10.1016/j.str.2017.12.006

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wj4"

@@ Line 1: / Line 1: @@
-==Crystal structure of the green fluorescent protein Clover mutant==
+==Crystal structure of redox-sensitive green fluorescent protein Clover mutant roClover1==
 <StructureSection load='5wj4' size='340' side='right' caption='[[5wj4]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wj4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wj4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ4 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wj4 OCA], [http://pdbe.org/5wj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wj4 RCSB], [http://www.ebi.ac.uk/pdbsum/5wj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wj4 ProSAT]</span></td></tr>
 </table>
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Aeqvi]]
 [[Category: Campbell, B C]]
 [[Category: Liu, C]]

5wj4

From Proteopedia

Revision as of 07:08, 31 January 2018

Crystal structure of redox-sensitive green fluorescent protein Clover mutant roClover1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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