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1mnn

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Revision as of 08:03, 31 January 2018

Structure of the sporulation specific transcription factor Ndt80 bound to DNA

Structural highlights

1mnn is a 3 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1mn4, 1m7u, 1m6u
Gene:	NDT80 (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NDT80_YEAST] Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Progression through the middle phase of sporulation in Saccharomyces cerevisiae is promoted by the successful completion of recombination at the end of prophase I. Completion of meiotic recombination allows the activation of the sporulation-specific transcription factor Ndt80, which binds to a specific DNA sequence, the middle sporulation element (MSE), and activates approximately 150 genes to enable progression through meiosis. Here, we isolate the DNA-binding domain of Ndt80 and determine its crystal structure both free and in complex with an MSE-containing DNA. The structure reveals that Ndt80 is a member of the Ig-fold family of transcription factors. The structure of the DNA-bound form, refined at 1.4 A, reveals an unexpected mode of recognition of 5'-pyrimidine- guanine-3' dinucleotide steps by arginine residues that simultaneously recognize the 3'-guanine base through hydrogen bond interactions and the 5'-pyrimidine through stacking/van der Waals interactions. Analysis of the DNA-binding affinities of MSE mutants demonstrates the central importance of these interactions, and of the AT-rich portion of the MSE. Functional similarities between Ndt80 and the Caenorhabditis elegans p53 homolog suggest an evolutionary link between Ndt80 and the p53 family.

Structure of the sporulation-specific transcription factor Ndt80 bound to DNA.,Lamoureux JS, Stuart D, Tsang R, Wu C, Glover JN EMBO J. 2002 Nov 1;21(21):5721-32. PMID:12411490^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xu L, Ajimura M, Padmore R, Klein C, Kleckner N. NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae. Mol Cell Biol. 1995 Dec;15(12):6572-81. PMID:8524222
↑ Chu S, Herskowitz I. Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80. Mol Cell. 1998 Apr;1(5):685-96. PMID:9660952
↑ Hepworth SR, Friesen H, Segall J. NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae. Mol Cell Biol. 1998 Oct;18(10):5750-61. PMID:9742092
↑ Pierce M, Benjamin KR, Montano SP, Georgiadis MM, Winter E, Vershon AK. Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression. Mol Cell Biol. 2003 Jul;23(14):4814-25. PMID:12832469
↑ Lamoureux JS, Stuart D, Tsang R, Wu C, Glover JN. Structure of the sporulation-specific transcription factor Ndt80 bound to DNA. EMBO J. 2002 Nov 1;21(21):5721-32. PMID:12411490

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mnn"

@@ Line 1: / Line 1: @@
 ==Structure of the sporulation specific transcription factor Ndt80 bound to DNA==
 <StructureSection load='1mnn' size='340' side='right' caption='[[1mnn]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mn4|1mn4]], [[1m7u|1m7u]], [[1m6u|1m6u]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NDT80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnn OCA], [http://pdbe.org/1mnn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mnn RCSB], [http://www.ebi.ac.uk/pdbsum/1mnn PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnn OCA], [http://pdbe.org/1mnn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mnn RCSB], [http://www.ebi.ac.uk/pdbsum/1mnn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnn ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mnn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mnn_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1mnn

From Proteopedia

Revision as of 08:03, 31 January 2018

Structure of the sporulation specific transcription factor Ndt80 bound to DNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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