6ey0
From Proteopedia
(Difference between revisions)
m (Protected "6ey0" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==N-terminal part (residues 30-212) of PorM with the llama naobody nb01== | |
| + | <StructureSection load='6ey0' size='340' side='right' caption='[[6ey0]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ey0]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EY0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EY0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ey0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ey0 OCA], [http://pdbe.org/6ey0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ey0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ey0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ey0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM. | ||
| - | + | Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.,Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. doi:, 10.1107/S2053230X17005969. Epub 2017 Apr 26. PMID:28471361<ref>PMID:28471361</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 6ey0" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Cambillau, C]] | [[Category: Cambillau, C]] | ||
[[Category: Leone, P]] | [[Category: Leone, P]] | ||
[[Category: Roche, J]] | [[Category: Roche, J]] | ||
| + | [[Category: Roussel, A]] | ||
| + | [[Category: Nanobody]] | ||
| + | [[Category: Protein transport]] | ||
Revision as of 06:23, 7 February 2018
N-terminal part (residues 30-212) of PorM with the llama naobody nb01
| |||||||||||
