Sandbox Reserved 1373
From Proteopedia
(Difference between revisions)
(→'Green Florescent Protein'==Your Heading Here (maybe something like 'Structure')) |
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{{Sandbox_Reserved_HLSC322}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | {{Sandbox_Reserved_HLSC322}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | ||
=='Green Florescent Protein'==Your Heading Here (maybe something like 'Structure')== | =='Green Florescent Protein'==Your Heading Here (maybe something like 'Structure')== | ||
| - | <StructureSection load='1bna' size='350' side='right' caption='Green Florescent Protein' scene=''> | ||
Green Florescent protein is a protein that converts the blue chemiluminescent of aequorin in the jellyfish into green fluorescent light. Because of it's green fluorescence, GFP can be utilized as a marker protein when incorporated into a variety of biological molecules and systems. This is useful for gene cloning, because it allows for researchers to determine from its use as a marker if their gene has been successfully incorporated into the cloning vector and replicated in their organism of choice. For example, if the gene coding for insulin was attached with the GFP, and the inserted into a cloning vector, the vector can then be electroporated into a fast growing organism like E.coli. After this, scientists can view if the gene was successfully incorporated based on if the ecoli is fluorescent or not. | Green Florescent protein is a protein that converts the blue chemiluminescent of aequorin in the jellyfish into green fluorescent light. Because of it's green fluorescence, GFP can be utilized as a marker protein when incorporated into a variety of biological molecules and systems. This is useful for gene cloning, because it allows for researchers to determine from its use as a marker if their gene has been successfully incorporated into the cloning vector and replicated in their organism of choice. For example, if the gene coding for insulin was attached with the GFP, and the inserted into a cloning vector, the vector can then be electroporated into a fast growing organism like E.coli. After this, scientists can view if the gene was successfully incorporated based on if the ecoli is fluorescent or not. | ||
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Regarding the structure, the protein is shaped like a barrel, which is from the folding of the <scene name='77/777693/Beta_pleated_sheets/1'>beta pleated sheets</scene> in the secondary structure. In addition, <scene name='77/777693/Alpha_helices/1'>alpha helix</scene> can be found running through the center of the barrel. | Regarding the structure, the protein is shaped like a barrel, which is from the folding of the <scene name='77/777693/Beta_pleated_sheets/1'>beta pleated sheets</scene> in the secondary structure. In addition, <scene name='77/777693/Alpha_helices/1'>alpha helix</scene> can be found running through the center of the barrel. | ||
</StructureSection>== | </StructureSection>== | ||
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<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | ||
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You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
Revision as of 21:52, 22 February 2018
| This Sandbox is Reserved from January through July 31, 2018 for use in the course HLSC322: Principles of Genetics and Genomics taught by Genevieve Houston-Ludlam at the University of Maryland, College Park, USA. This reservation includes Sandbox Reserved 1311 through Sandbox Reserved 1430. |
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'Green Florescent Protein'==Your Heading Here (maybe something like 'Structure')
Green Florescent protein is a protein that converts the blue chemiluminescent of aequorin in the jellyfish into green fluorescent light. Because of it's green fluorescence, GFP can be utilized as a marker protein when incorporated into a variety of biological molecules and systems. This is useful for gene cloning, because it allows for researchers to determine from its use as a marker if their gene has been successfully incorporated into the cloning vector and replicated in their organism of choice. For example, if the gene coding for insulin was attached with the GFP, and the inserted into a cloning vector, the vector can then be electroporated into a fast growing organism like E.coli. After this, scientists can view if the gene was successfully incorporated based on if the ecoli is fluorescent or not.
Regarding the structure, the protein is shaped like a barrel, which is from the folding of the in the secondary structure. In addition, can be found running through the center of the barrel. </StructureSection>==
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References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
