2euw
From Proteopedia
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|PDB= 2euw |SIZE=350|CAPTION= <scene name='initialview01'>2euw</scene>, resolution 1.680Å | |PDB= 2euw |SIZE=350|CAPTION= <scene name='initialview01'>2euw</scene>, resolution 1.680Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= NDT80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= NDT80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1mnn|1MNN]], [[1mn4|1MN4]], [[1m6u|1M6U]], [[1m7u|1M7U]], [[2etw|2ETW]], [[2euv|2EUV]], [[2eux|2EUX]], [[2euz|2EUZ]], [[2evf|2EVF]], [[2evg|2EVG]], [[2evh|2EVH]], [[2ehi|2EHI]], [[2ehj|2EHJ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2euw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2euw OCA], [http://www.ebi.ac.uk/pdbsum/2euw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2euw RCSB]</span> | ||
}} | }} | ||
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[[Category: ig-fold transcription factor]] | [[Category: ig-fold transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:54:03 2008'' |
Revision as of 23:54, 30 March 2008
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| , resolution 1.680Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | NDT80 (Saccharomyces cerevisiae) | ||||||
| Related: | 1MNN, 1MN4, 1M6U, 1M7U, 2ETW, 2EUV, 2EUX, 2EUZ, 2EVF, 2EVG, 2EVH, 2EHI, 2EHJ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of a Ndt80-DNA complex (MSE mutant mA4T)
Overview
The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.
About this Structure
2EUW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:16531239
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